ICCAS OpenIR
Combination of DNA Ligase Reaction and Gold Nanoparticle-Quenched Fluorescent Oligonucleotides: A Simple and Efficient Approach for Fluorescent Assaying of Single-Nucleotide Polymorphisms
Wang, Hao1,2; Li, Jishan1; Wang, Yongxiang1,2; Jin, Jiangyu1,2; Yang, Ronghua1; Wang, Kemin1; Tan, Weihong1
2010-09-15
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume82Issue:18Pages:7684-7690
AbstractA new fluorescent sensing approach for detection of single-nucleotide polymorphisms (SNPs) is proposed based on the ligase reaction and gold nanoparticle (AuNPs)-quenched fluorescent oligonucleotides. The design exploits the strong fluorescence quenching of AuNPs for organic dyes and the difference in noncovalent interactions of the nanoparticles with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA), where ssDNA can be adsorbed onto the surface of AuNPs while dsDNA cannot be. In the assay, two half primer DNA probes, one being labeled with a dye and the other being phosphorylated, were first incubated with a target DNA template. In the presence of DNA ligase, the two captured ssDNAs are linked for the perfectly matched DNA target to form a stable duplex, but the duplex could not be formed by the single-base mismatched DNA template. After addition of AuNPs, the fluorescence of dye-tagged DNA probe will be efficiently quenched unless the perfectly matched DNA target is present. To demonstrate the feasibility of this design, the performance of SNP detection using two different DNA ligases, T4 DNA ligase and Eschcrichia coli DNA ligase, were investigated. In the case of T4 DNA ligase, the signal enhancement of the dye-tagged DNA for perfectly matched DNA target is 4.6-fold higher than that for the single-base mismatched DNA. While in the presence of E. coli DNA ligase, the value raises to be 30.2, suggesting excellent capability for SNP discrimination.
DOI10.1021/ac1015031
Indexed BySCI
Language英语
WOS IDWOS:000281710900023
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:52[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/71128
Collection中国科学院化学研究所
Corresponding AuthorYang, Ronghua
Affiliation1.Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Coll Biol, Changsha 410082, Hunan, Peoples R China
2.Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Wang, Hao,Li, Jishan,Wang, Yongxiang,et al. Combination of DNA Ligase Reaction and Gold Nanoparticle-Quenched Fluorescent Oligonucleotides: A Simple and Efficient Approach for Fluorescent Assaying of Single-Nucleotide Polymorphisms[J]. ANALYTICAL CHEMISTRY,2010,82(18):7684-7690.
APA Wang, Hao.,Li, Jishan.,Wang, Yongxiang.,Jin, Jiangyu.,Yang, Ronghua.,...&Tan, Weihong.(2010).Combination of DNA Ligase Reaction and Gold Nanoparticle-Quenched Fluorescent Oligonucleotides: A Simple and Efficient Approach for Fluorescent Assaying of Single-Nucleotide Polymorphisms.ANALYTICAL CHEMISTRY,82(18),7684-7690.
MLA Wang, Hao,et al."Combination of DNA Ligase Reaction and Gold Nanoparticle-Quenched Fluorescent Oligonucleotides: A Simple and Efficient Approach for Fluorescent Assaying of Single-Nucleotide Polymorphisms".ANALYTICAL CHEMISTRY 82.18(2010):7684-7690.
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