ICCAS OpenIR
A simple and rapid competitive enzyme-linked immunosorbent assay (cELISA) for high-throughput measurement of secretory immunoglobulin A (sIgA) in saliva
Wang, Dan; Zhuo, Jun-Qiao; Zhao, Mei-Ping
2010-06-30
Source PublicationTALANTA
ISSN0039-9140
Volume82Issue:1Pages:432-436
AbstractA simple competitive enzyme-linked immunosorbent assay (cELISA) was established for rapid measurement of secretory immunoglobulin A (sIgA) in saliva. The method was based on competitive reaction between the immobilized IgA and free IgA in the solution for the limited amount of horseradish peroxidase-conjugated rabbit anti-human IgA. In comparison with the conventionally used Sandwich ELISA, the cELISA is simpler, low-cost, and shows better reproducibility since it is not affected by the variation of capture antibodies from different batches. The assay time was also significantly reduced from more than 5 h to less than 3 h. Different curve-fitting models were compared, among which the fully specified logit-log model gave the best results. The linear working range and limit of detection were found to be 0.1-100 mu g mL(-1) and 0.05 mu g mL(-1), respectively. Matrix effects of saliva samples were investigated and a reasonable range of dilution factors were proposed. The developed method offers a very practical approach for high-throughput measurement of sIgA in saliva samples. (C) 2010 Elsevier B.V. All rights reserved.
KeywordImmunoglobulin a (Iga) Saliva Sample Competitive Enzyme-linked Immunosorbent Assay (Celisa)
DOI10.1016/j.talanta.2010.04.040
Indexed BySCI
Language英语
WOS IDWOS:000279488900063
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/71060
Collection中国科学院化学研究所
Corresponding AuthorZhao, Mei-Ping
AffiliationPeking Univ, Coll Chem & Mol Engn, Minist Educ, Beijing Natl Lab Mol Sci,Key Lab Bioorgan Chem &, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Wang, Dan,Zhuo, Jun-Qiao,Zhao, Mei-Ping. A simple and rapid competitive enzyme-linked immunosorbent assay (cELISA) for high-throughput measurement of secretory immunoglobulin A (sIgA) in saliva[J]. TALANTA,2010,82(1):432-436.
APA Wang, Dan,Zhuo, Jun-Qiao,&Zhao, Mei-Ping.(2010).A simple and rapid competitive enzyme-linked immunosorbent assay (cELISA) for high-throughput measurement of secretory immunoglobulin A (sIgA) in saliva.TALANTA,82(1),432-436.
MLA Wang, Dan,et al."A simple and rapid competitive enzyme-linked immunosorbent assay (cELISA) for high-throughput measurement of secretory immunoglobulin A (sIgA) in saliva".TALANTA 82.1(2010):432-436.
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