ICCAS OpenIR
Kinetic Study on the Reentrant Condensation of Oligonucleotide in Trivalent Salt Solution
Zhou, Jihan; Ke, Fuyou1; Liang, Dehai
2010-11-04
Source PublicationJOURNAL OF PHYSICAL CHEMISTRY B
ISSN1520-6106
Volume114Issue:43Pages:13675-13680
AbstractThe reentrant condensation of 21-bp oligonucleotide in the presence of spermidine was investigated by laser light scattering and capillary electrophoresis. 21-bp oligonucleotide showed a bimodal distribution in 1 x TE buffer, with the slow mode being the characteristic diffusion of polyelectrolyte in solution without enough salt. At the fixed spermidine concentration, the reentry of oligonucleotide underwent aggregation, phase separation, and disassociation in sequence with time, and the kinetics was extremely slow. For example, it took more than 1200 h (50 days) for the reentry to complete at 21 mM spermidine. Higher spermidine concentration led to faster kinetics. After reentry, the slow mode disappeared, and the charges of oligonucleotide were at least partially neutralized. No prominent charge inversion was observed. The kinetics of oligonucleotide reentry in the presence of spermidine gained insight in the interactions of polyelectrolyte in aqueous solution.
DOI10.1021/jp1074187
Indexed BySCI
Language英语
WOS IDWOS:000283519600008
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/71038
Collection中国科学院化学研究所
Corresponding AuthorKe, Fuyou
Affiliation1.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
2.Peking Univ, Coll Chem & Mol Engn, Key Lab Polymer Chem & Phys, Minist Educ, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Jihan,Ke, Fuyou,Liang, Dehai. Kinetic Study on the Reentrant Condensation of Oligonucleotide in Trivalent Salt Solution[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2010,114(43):13675-13680.
APA Zhou, Jihan,Ke, Fuyou,&Liang, Dehai.(2010).Kinetic Study on the Reentrant Condensation of Oligonucleotide in Trivalent Salt Solution.JOURNAL OF PHYSICAL CHEMISTRY B,114(43),13675-13680.
MLA Zhou, Jihan,et al."Kinetic Study on the Reentrant Condensation of Oligonucleotide in Trivalent Salt Solution".JOURNAL OF PHYSICAL CHEMISTRY B 114.43(2010):13675-13680.
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