ICCAS OpenIR
Study on Intercalations between Double-Stranded DNA and Pyrene by Single-Molecule Force Spectroscopy: Toward the Detection of Mismatch in DNA
Jiang, Zhenhua1; Zhang, Yiheng1; Yu, Ying1; Wang, Zhiqiang1; Zhang, Xi1; Duan, Xinrui2; Wang, Shu2
2010-09-07
Source PublicationLANGMUIR
ISSN0743-7463
Volume26Issue:17Pages:13773-13777
AbstractIntercalation interactions between planar aromatic molecules and double-stranded DNA (dsDNA) relate to not only the structure of the guest molecules but also the structure of the dsDNA. In this letter, we have comparatively studied the intercalation between pyrene and fully matched or mismatched dsDNA using single-molecule force spectroscopy (SMFS). The significant difference in rupture forces, upon pyrene unbinding from 25-mer dsDNA with or without mismatches, is observed at the single-molecule level, indicating the influence of mismatches on the interaction between pyrene and dsDNA. In the analysis of the dynamic force spectra, two transition barriers are revealed for pyrene unbinding from matched sites in dsDNA and for pyrene unbinding from mismatched sites as well. These results suggest that SMFS is a useful single-molecule method for the detection of mismatches in dsDNA by the intercalation of pyrene.
DOI10.1021/la102647p
Indexed BySCI
Language英语
WOS IDWOS:000281354000012
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/70808
Collection中国科学院化学研究所
Corresponding AuthorZhang, Xi
Affiliation1.Tsinghua Univ, Dept Chem, Key Lab Organ Optoelect & Mol Engn, Beijing 100084, Peoples R China
2.Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing 100084, Peoples R China
Recommended Citation
GB/T 7714
Jiang, Zhenhua,Zhang, Yiheng,Yu, Ying,et al. Study on Intercalations between Double-Stranded DNA and Pyrene by Single-Molecule Force Spectroscopy: Toward the Detection of Mismatch in DNA[J]. LANGMUIR,2010,26(17):13773-13777.
APA Jiang, Zhenhua.,Zhang, Yiheng.,Yu, Ying.,Wang, Zhiqiang.,Zhang, Xi.,...&Wang, Shu.(2010).Study on Intercalations between Double-Stranded DNA and Pyrene by Single-Molecule Force Spectroscopy: Toward the Detection of Mismatch in DNA.LANGMUIR,26(17),13773-13777.
MLA Jiang, Zhenhua,et al."Study on Intercalations between Double-Stranded DNA and Pyrene by Single-Molecule Force Spectroscopy: Toward the Detection of Mismatch in DNA".LANGMUIR 26.17(2010):13773-13777.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Jiang, Zhenhua]'s Articles
[Zhang, Yiheng]'s Articles
[Yu, Ying]'s Articles
Baidu academic
Similar articles in Baidu academic
[Jiang, Zhenhua]'s Articles
[Zhang, Yiheng]'s Articles
[Yu, Ying]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Jiang, Zhenhua]'s Articles
[Zhang, Yiheng]'s Articles
[Yu, Ying]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.