DNA Electrochemical Biosensor for Trace Hg2+ Detection
Ge Fang1,2; Cao Rui-Guo1; Zhu Bin1; Li Jing-Jian1; Xu Dong-Sheng1
AbstractIn this paper we demonstrated a novel type of electrochemical Hg2+ biosensor based on a DNA-modified electrode. Ferrocenyl. modified T. rich DNA (DNA-Fc) molecules were synthesized for use as electrochemical probes. We then fixed these DNA-Fc probes onto a gold electrode surface by self. assembly. In the presence of Hg2+, the single strand DNA on the electrode surface turned to a thymine-Hg2+-thymine (T-Hg2+-T) hairpin structure. The ferrocenyl groups were kept away from the surface of the electrode, and this could be measured sensitively by differential pulse voltammetry (DPV). The results show a reduction peak of ferrocene at 0.26 V (vs saturated calomel electrode (SCE)) and the peak current of DPV decreased with increasing the concentration of Hg2+. The rate of current change is linear with regards to lgc(Hg2+) over a concentration range from 0.1 nmol . L-1 to 1 mu mol . L-1 and with a detection limit of 0.1 mol . L-1. A test for interference metal ions showed that this electrochemical biosensor based on a DNA modified electrode is highly sensitive and selective, and it can be widely used for trace Hg2+ detection.
KeywordBiosensor Hg2++++ Dna Conformational Change Differential Pulse Voltammetry Electrochemical Impedance Spectroscopy
Indexed BySCI
WOS IDWOS:000279776400008
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Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorLi Jing-Jian
Affiliation1.Peking Univ, Coll Chem & Mol Engn, State Key Lab Struct Chem Unstable & Stable Speci, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
2.Coll Sanming, Dept Chem & Biol Engn, Sanming 365004, Fujian Province, Peoples R China
Recommended Citation
GB/T 7714
Ge Fang,Cao Rui-Guo,Zhu Bin,et al. DNA Electrochemical Biosensor for Trace Hg2+ Detection[J]. ACTA PHYSICO-CHIMICA SINICA,2010,26(7):1779-1783.
APA Ge Fang,Cao Rui-Guo,Zhu Bin,Li Jing-Jian,&Xu Dong-Sheng.(2010).DNA Electrochemical Biosensor for Trace Hg2+ Detection.ACTA PHYSICO-CHIMICA SINICA,26(7),1779-1783.
MLA Ge Fang,et al."DNA Electrochemical Biosensor for Trace Hg2+ Detection".ACTA PHYSICO-CHIMICA SINICA 26.7(2010):1779-1783.
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