ICCAS OpenIR
Methanol as a probe to investigate the relationship between the secondary electron donor Tyr(Z) and the substrate water molecules in active photosystem II
Bao Han; Zhang ChunXi; Ren YaNan; Zhao JingQuan
2010-03-01
Source PublicationCHINESE SCIENCE BULLETIN
ISSN1001-6538
Volume55Issue:9Pages:809-813
AbstractThe secondary electron donor, Tyr(Z), is implicated in tuning the primary charge separation and the water oxidation in active photosystem 11 (PSII). Two types of mechanisms have been proposed to explain the function of Tyr(Z). One is that Tyr(Z) tunes the water oxidation through the direct interaction with substrate water rnolecules the other is that Tyr(Z) is located in a hydrophobic environment without interacting with H2O, and controls the water oxidation by tuning the strength of the hydrogen bond between Tyr(Z) and His190. Here, methanol was used as a probe to study the possible relationship between Tyr(Z) and H2O by monitoring the Tyr(Z) oxidation and Tyr(Z)* reduction at cryogenic temperatures with electron paramagnetic resonance spectroscopy. The oxidation of Tyr(Z) and reduction of Tyr(Z)* in both S-2 and S-0 states at 10 K were accelerated by addition of a small amount of methanol (6%). Theoretical studies indicate that Tyr oxidation becomes more difficult if it interacts directly with the methanol molecule; while the decrease of the polarity of its environment accelerates the oxidation of Tyr. Accordingly, CH3OH does not directly interact with Tyr(Z) in active PSII, and the accelerative effect of methanol is caused by the strength increase of the hydrogen bond between Tyr(Z) and His 190, resulting from the decrease of polarity of their environment after the displacement of H2O by CH3OH inside PSII Considering the similarity between methanol and water, the results in this study support the model in which Tyr(Z) does not interact with H2O in active PSII.
KeywordPhotosystem Ii Secondary Electron Donor Tyr(z) Methanol Water Electron Paramagnetic Resonance
DOI10.1007/s11434-010-0047-0
Indexed BySCI
Language英语
WOS IDWOS:000275821800005
PublisherSCIENCE PRESS
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/70588
Collection中国科学院化学研究所
Corresponding AuthorZhang ChunXi
AffiliationChinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Lab Photochem, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Bao Han,Zhang ChunXi,Ren YaNan,et al. Methanol as a probe to investigate the relationship between the secondary electron donor Tyr(Z) and the substrate water molecules in active photosystem II[J]. CHINESE SCIENCE BULLETIN,2010,55(9):809-813.
APA Bao Han,Zhang ChunXi,Ren YaNan,&Zhao JingQuan.(2010).Methanol as a probe to investigate the relationship between the secondary electron donor Tyr(Z) and the substrate water molecules in active photosystem II.CHINESE SCIENCE BULLETIN,55(9),809-813.
MLA Bao Han,et al."Methanol as a probe to investigate the relationship between the secondary electron donor Tyr(Z) and the substrate water molecules in active photosystem II".CHINESE SCIENCE BULLETIN 55.9(2010):809-813.
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