ICCAS OpenIR
Synthesis and characterization of multifunctional poly(glycidyl methacrylate) microspheres and their use in cell separation
Dong, Xiaoqing1,2; Zheng, Yonghui1; Huang, Yubin1; Chen, Xuesi1; Jing, Xiabin1
2010-10-15
Source PublicationANALYTICAL BIOCHEMISTRY
ISSN0003-2697
Volume405Issue:2Pages:207-212
AbstractMultifunctional poly(glycidyl methacrylate) (PGMA) microspheres containing magnetic, fluorescent, and cancer cell-specific moieties were prepared in four steps: (i) preparation of parent PGMA microspheres by dispersion polymerization and their reaction with ethylenediamine to obtain amino groups, (ii) precipitation of iron ions (Fe(2+) and Fe(3+)) to form Fe(3)O(4) nanoparticles within the microspheres, (iii) consecutive reactions of folic acid with the amino groups on PGMA, and (iv) incorporation of fluorescein isothiocyanate into the microspheres. The microspheres were superparamagnetic, highly monodispersive, intensively fluorescent, and capable of recognizing and binding cancer cells that overexpress folic acid receptors. It was demonstrated that with these microspheres, HeLa cells could be captured from their suspension and easily moved in the direction of the externally applied magnetic field. (C) 2010 Elsevier Inc. All rights reserved.
KeywordMicrospheres Fe(3)o(4) Nanoparticles Fluorescence Folic Acid Cell Detection And Separation
DOI10.1016/j.ab.2010.06.022
Indexed BySCI
Language英语
WOS IDWOS:000281296300009
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
Citation statistics
Cited Times:37[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/70420
Collection中国科学院化学研究所
Corresponding AuthorJing, Xiabin
Affiliation1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Dong, Xiaoqing,Zheng, Yonghui,Huang, Yubin,et al. Synthesis and characterization of multifunctional poly(glycidyl methacrylate) microspheres and their use in cell separation[J]. ANALYTICAL BIOCHEMISTRY,2010,405(2):207-212.
APA Dong, Xiaoqing,Zheng, Yonghui,Huang, Yubin,Chen, Xuesi,&Jing, Xiabin.(2010).Synthesis and characterization of multifunctional poly(glycidyl methacrylate) microspheres and their use in cell separation.ANALYTICAL BIOCHEMISTRY,405(2),207-212.
MLA Dong, Xiaoqing,et al."Synthesis and characterization of multifunctional poly(glycidyl methacrylate) microspheres and their use in cell separation".ANALYTICAL BIOCHEMISTRY 405.2(2010):207-212.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Dong, Xiaoqing]'s Articles
[Zheng, Yonghui]'s Articles
[Huang, Yubin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Dong, Xiaoqing]'s Articles
[Zheng, Yonghui]'s Articles
[Huang, Yubin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Dong, Xiaoqing]'s Articles
[Zheng, Yonghui]'s Articles
[Huang, Yubin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.