ICCAS OpenIR
Simple and highly sensitive detection of hepatotoxin microcystin-LR via colorimetric variation based on polydiacetylene vesicles
Xia, Yuetong; Deng, Jieli; Jiang, Long
2010-03-19
Source PublicationSENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
Volume145Issue:2Pages:713-719
AbstractA highly sensitive and simple approach for detecting hepatotoxin microcystin-LR (MC-LR) was developed based on color-changeable polydiacetylene (PDA) vesicles. By an EDC/NHS reaction, we successfully embedded a monoclonal antibody (mAb) of MC-LR (anti-MC-LR) onto the PDA vesicle surfaces, and a MC-LR recognition vesicle (PDA-anti-MC-LR) was formed. The optimal concentration for anti-MC-LR was determined that would guarantee the PDA immunosensor to exert its highest recognition effect. Upon application of MC-LR, a specific immunoreaction took place, which altered the PDA conformation and led to a color change. The lowest detection limit could be 1 ng/mL - the maximum concentration recommended by WHO in drinking water for humans. Although immunoassays have found applications in medical diagnostics and therapy, the procedures commonly used nowadays are too complicated. This chromatic immunosensor based on PDA reported in this paper has the advantage of high sensitivity, rapidity, cost-effectiveness, and convenience for MC-LR detection. Not only it is more adaptable for on-the-spot water detection, compared with current MC-LR detecting techniques, but the methodology here ensured optimal embedding of antibody to the PDA vesicles of great significance for fabrication of other nanosensors. This technology, based on antibody-antigen interaction, and its broadened use in PDA biosensors, has potential application in many fields. (C) 2010 Elsevier BM. All rights reserved.
KeywordCyanotoxin Surface Water Immunoassay Polydiacetylene Color Change
DOI10.1016/j.snb.2010.01.029
Indexed BySCI
Language英语
WOS IDWOS:000276589900017
PublisherELSEVIER SCIENCE SA
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/70266
Collection中国科学院化学研究所
Corresponding AuthorJiang, Long
AffiliationChinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Xia, Yuetong,Deng, Jieli,Jiang, Long. Simple and highly sensitive detection of hepatotoxin microcystin-LR via colorimetric variation based on polydiacetylene vesicles[J]. SENSORS AND ACTUATORS B-CHEMICAL,2010,145(2):713-719.
APA Xia, Yuetong,Deng, Jieli,&Jiang, Long.(2010).Simple and highly sensitive detection of hepatotoxin microcystin-LR via colorimetric variation based on polydiacetylene vesicles.SENSORS AND ACTUATORS B-CHEMICAL,145(2),713-719.
MLA Xia, Yuetong,et al."Simple and highly sensitive detection of hepatotoxin microcystin-LR via colorimetric variation based on polydiacetylene vesicles".SENSORS AND ACTUATORS B-CHEMICAL 145.2(2010):713-719.
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