ICCAS OpenIR
A protein interaction network for the analysis of the neuronal differentiation of neural stem cells in response to titanium dioxide nanoparticles
Liu, Xiaoyan1; Ren, Xiufang1; Deng, Xiaoyong2; Huo, Yinan1; Xie, Jiang4; Huang, Hai1; Jiao, Zheng2; Wu, Minghong2; Liu, Yuanfang2,3; Wen, Tieqiao1,5
2010-04-01
Source PublicationBIOMATERIALS
ISSN0142-9612
Volume31Issue:11Pages:3063-3070
AbstractThe effects of titanium dioxide (TiO(2)) nanoparticles (NPs) on the differentiation of neural stem cells are reported. Our findings indicate that TiO(2) NPs lead to a differentiational tendency towards neurons from neural stem cells, suggesting TiO(2) NPs might be a beneficial inducer for neuronal differentiation. To insight into the possible molecular mechanism of the neuronal differentiation, we conducted a protein-protein interaction network (PIN) analysis. To this end, a global mapping of target proteins induced by TiO(2) NPs was first made by a 2-dimensional electrophoresis analysis. Results showed that 9 proteins were significantly changed and then they were subjected to the mass spectrometric assay. All 9 identified proteins are involved in signal, molecular chaperones, cytoskeleton, and nucleoprotein. Further, based on our experimental data and DIP, IntAct-EBI, GRID database, a protein-protein interaction network was constructed, which provides highly integrated information exhibiting the protein-protein interaction. By analysis of the gene expression, the signal pathway involving Cx43 phosphotylation, which is negatively regulated by the protein kinase C epsilon (PKC epsilon), is demonstrated. It is inferred that PKCE plays a pivotal negative role in the neuronal differentiation of stem neural cells in response to the TiO(2) NPs exposure. (C) 2009 Elsevier Ltd. All rights reserved.
KeywordTitanium Dioxide Nanoparticles Neural Stem Cells Differentiation Protein-protein Interaction Proteomics
DOI10.1016/j.biomaterials.2009.12.054
Indexed BySCI
Language英语
WOS IDWOS:000275946500013
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:35[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/70260
Collection中国科学院化学研究所
Corresponding AuthorWen, Tieqiao
Affiliation1.Shanghai Univ, Sch Life Sci, Mol Neurobiol Lab, Shanghai 200444, Peoples R China
2.Shanghai Univ, Inst Nanochem & Nanobiol, Shanghai 200444, Peoples R China
3.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
4.Shanghai Univ, Sch Comp Engn & Sci, Shanghai 200072, Peoples R China
5.Shanghai Univ, Inst Syst Biol, Shanghai 200444, Peoples R China
Recommended Citation
GB/T 7714
Liu, Xiaoyan,Ren, Xiufang,Deng, Xiaoyong,et al. A protein interaction network for the analysis of the neuronal differentiation of neural stem cells in response to titanium dioxide nanoparticles[J]. BIOMATERIALS,2010,31(11):3063-3070.
APA Liu, Xiaoyan.,Ren, Xiufang.,Deng, Xiaoyong.,Huo, Yinan.,Xie, Jiang.,...&Wen, Tieqiao.(2010).A protein interaction network for the analysis of the neuronal differentiation of neural stem cells in response to titanium dioxide nanoparticles.BIOMATERIALS,31(11),3063-3070.
MLA Liu, Xiaoyan,et al."A protein interaction network for the analysis of the neuronal differentiation of neural stem cells in response to titanium dioxide nanoparticles".BIOMATERIALS 31.11(2010):3063-3070.
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