ICCAS OpenIR
High-performance affinity monolith chromatography for chiral separation and determination of enzyme kinetic constants
Yao, Chunhe1,3; Qi, Li1; Qiao, Juan1,3; Zhang, Haizhi1,3; Wang, Fuyi1; Chen, Yi1; Yang, Gengliang2
2010-09-15
Source PublicationTALANTA
ISSN0039-9140
Volume82Issue:4Pages:1332-1337
AbstractA new kind of immobilized human serum albumin (HSA) column was developed by using the sub-micron skeletal polymer monolith based on poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) [poly(GMA-EDMA)] as the support of high-performance affinity chromatography. Using the epoxide functional groups presented in GMA, the HSA immobilization procedure was performed by two different means. The affinity columns were successfully adopted for the chiral separation of D,L-amino acids (AAs). Then this method was shown to be applicable to the quantitative analysis of D-tryptophan, with a linear ;range between 12.0 mu M and 979.0 mu M, and a correlation coefficient above 0.99. Furthermore, it was used for the analysis of urine sample. This assay is demonstrated to be facile and relatively rapid. So it allows us to measure the enzyme catalytic activity in the incubation of D,L-AAs with D-AA oxidase and to study the kinetics of the enzyme reaction. It implied that the affinity monolithic columns can be a useful tool for studying DAAO enzyme reaction and investigating the potential enzyme mechanism requirement among chiral conversion. (C) 2010 Elsevier B.V. All rights reserved.
KeywordHigh-performance Affinity Chromatography Human Serum Albumin Enzyme Kinetic Study Chiral Separation Monolithic Column
DOI10.1016/j.talanta.2010.06.041
Indexed BySCI
Language英语
WOS IDWOS:000282480500036
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:30[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/70028
Collection中国科学院化学研究所
Corresponding AuthorQi, Li
Affiliation1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
2.Hebei Univ, Coll Pharm, Baoding 071002, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yao, Chunhe,Qi, Li,Qiao, Juan,et al. High-performance affinity monolith chromatography for chiral separation and determination of enzyme kinetic constants[J]. TALANTA,2010,82(4):1332-1337.
APA Yao, Chunhe.,Qi, Li.,Qiao, Juan.,Zhang, Haizhi.,Wang, Fuyi.,...&Yang, Gengliang.(2010).High-performance affinity monolith chromatography for chiral separation and determination of enzyme kinetic constants.TALANTA,82(4),1332-1337.
MLA Yao, Chunhe,et al."High-performance affinity monolith chromatography for chiral separation and determination of enzyme kinetic constants".TALANTA 82.4(2010):1332-1337.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yao, Chunhe]'s Articles
[Qi, Li]'s Articles
[Qiao, Juan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yao, Chunhe]'s Articles
[Qi, Li]'s Articles
[Qiao, Juan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yao, Chunhe]'s Articles
[Qi, Li]'s Articles
[Qiao, Juan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.