ICCAS OpenIR
Enhancement of TAT cell membrane penetration efficiency by dimethyl sulphoxide
Wang, Hu1; Zhong, Chun-Yan1; Wu, Jiang-Feng1; Huang, Yu-Bin2; Liu, Chang-Bai1
2010-04-02
Source PublicationJOURNAL OF CONTROLLED RELEASE
ISSN0168-3659
Volume143Issue:1Pages:64-70
AbstractCell penetrating peptides (CPPs) are promising tools for transducing presynthesized therapeutic molecules which possess low membrane permeability. The poor efficiency of cellular uptake and unexpected cellular localization are still the main obstacles to the development of drug delivery by using CPPs. In this study, we investigated the effect of a penetration enhancer, dimethylsulfoxide (DMSO), on the penetrating efficiency of a synthetic TAT peptide or the TAT fusion protein. FITC-labeled TAT and TAT-GFP were added to 10% DMSO or 100 mu M chloroquine pretreated cells, fluorescence uptake into culturing cells was observed using fluorescence microscopy, FACS or quantitatively analyzed by a fluorescence spectrum. 10% DMSO treatment markedly increased internalization of TAT into cells and appeared in a well-distributed pattern throughout the cytosol and nucleus without membrane perforating or detectable cytotoxicity, the enhancement effect by 10% DMSO was reduced by endocytosis inhibitors including ammonium chloride and sodium azide. 10% DMSO also enhanced TAT-Apoptin induced apoptosis of carcinoma cells. These findings implicated that DMSO can be a novel delivery enhancer appropriate for CPP penetration. (C) 2009 Elsevier B.V. All rights reserved.
KeywordCell Penetrating Peptides (Cpps) Dimethylsulfoxide (Dmso) Tat Penetration
DOI10.1016/j.jconrel.2009.12.003
Indexed BySCI
Language英语
WOS IDWOS:000276766600009
PublisherELSEVIER SCIENCE BV
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/69988
Collection中国科学院化学研究所
Corresponding AuthorLiu, Chang-Bai
Affiliation1.Three Georges Univ, Inst Mol Biol, Yichang 443002, Peoples R China
2.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China
Recommended Citation
GB/T 7714
Wang, Hu,Zhong, Chun-Yan,Wu, Jiang-Feng,et al. Enhancement of TAT cell membrane penetration efficiency by dimethyl sulphoxide[J]. JOURNAL OF CONTROLLED RELEASE,2010,143(1):64-70.
APA Wang, Hu,Zhong, Chun-Yan,Wu, Jiang-Feng,Huang, Yu-Bin,&Liu, Chang-Bai.(2010).Enhancement of TAT cell membrane penetration efficiency by dimethyl sulphoxide.JOURNAL OF CONTROLLED RELEASE,143(1),64-70.
MLA Wang, Hu,et al."Enhancement of TAT cell membrane penetration efficiency by dimethyl sulphoxide".JOURNAL OF CONTROLLED RELEASE 143.1(2010):64-70.
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