ICCAS OpenIR
Monomeric type I and type III transforming growth factor-beta receptors and their dimerization revealed by single- molecule imaging
Zhang, Wei1; Yuan, Jinghe1; Yang, Yong1; Xu, Li1; Wang, Qiang2; Zuo, Wei2; Fang, Xiaohong1; Chen, Ye-Guang2
2010-11-01
Source PublicationCELL RESEARCH
ISSN1001-0602
Volume20Issue:11Pages:1216-1223
AbstractTransforming growth factor-beta (TGF-beta) binds with two transmembrane serine/threonine kinase receptors, type II (T beta RII) and type I receptors (T beta RI), and one accessory receptor, type III receptor (T beta RIII), to transduce signals across cell membranes. Previous biochemical studies suggested that T beta RI and T beta RIII are preexisted homo-dimers. Using single-molecule microscopy to image green fluorescent protein-labeled membrane proteins, for the first time we have demonstrated that T beta RI and T beta RIII could exist as monomers at a low expression level. Upon TGF-beta 1 stimulation, T beta RI follows the general ligand-induced receptor dimerization model for activation, but this process is T beta RII-dependent. The monomeric status of the non-kinase receptor T beta RIII is unchanged in the presence of TGF-beta 1. With the increase of receptor expression, both T beta RI and T beta RIII can be assembled into dimers on cell surfaces.
KeywordSingle-molecule Fluorescence Tgf-beta Signaling Type i Tgf-beta Receptor Type Iii Tgf-beta Receptor Subunit Stoichiometry
DOI10.1038/cr.2010.105
Indexed BySCI
Language英语
WOS IDWOS:000283713000007
PublisherINST BIOCHEMISTRY & CELL BIOLOGY
Citation statistics
Cited Times:38[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/69680
Collection中国科学院化学研究所
Corresponding AuthorFang, Xiaohong
Affiliation1.Chinese Acad Sci, Key Lab Mol Nanostruct & Nanotechnol, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
2.Tsinghua Univ, Sch Life Sci, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Wei,Yuan, Jinghe,Yang, Yong,et al. Monomeric type I and type III transforming growth factor-beta receptors and their dimerization revealed by single- molecule imaging[J]. CELL RESEARCH,2010,20(11):1216-1223.
APA Zhang, Wei.,Yuan, Jinghe.,Yang, Yong.,Xu, Li.,Wang, Qiang.,...&Chen, Ye-Guang.(2010).Monomeric type I and type III transforming growth factor-beta receptors and their dimerization revealed by single- molecule imaging.CELL RESEARCH,20(11),1216-1223.
MLA Zhang, Wei,et al."Monomeric type I and type III transforming growth factor-beta receptors and their dimerization revealed by single- molecule imaging".CELL RESEARCH 20.11(2010):1216-1223.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Wei]'s Articles
[Yuan, Jinghe]'s Articles
[Yang, Yong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Wei]'s Articles
[Yuan, Jinghe]'s Articles
[Yang, Yong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Wei]'s Articles
[Yuan, Jinghe]'s Articles
[Yang, Yong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.