ICCAS OpenIR
Inspection into the Interaction of Bovine Serum Albumin with Gold Nanoparticles by Capillary Electrophoresis
Xie Ming-Yi1; Guo Zhen-Peng1; Chen Yi1,2
2010-11-10
Source PublicationCHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
ISSN0251-0790
Volume31Issue:11Pages:2162-2166
AbstractA capillary electrophoretic method was developed to study the interaction of gold nanoparticles (GNPs) with bovine serum albumin(BSA), capable of determining the binding constant and binding capacity. For demonstration citric acid-protected GNPs were tried, the measured bonding constant was 28.6 L/mu mol and the saturated binding capacity was 24 BSA molecules per GNP, corresponding to a surface concentration of 4.5 x 10(12) BSA/cm(2). The determination was shown to depend on the incubating pH value and the concentration of run buffer which should be optimized accordingly. It was found that the peaks of the citric acid-protected GNPs were commonly broad, overlaid with irreproducible bars, but could easily be improved by addition of a small amount of BSA. The established method is expected to be extendable to inspecting the interaction of GNPs with other biomolecules.
KeywordCapillary Electrophoresis Gold Nanoparticle Bovine Serum Albumin Interaction Constant
Indexed BySCI
Language英语
WOS IDWOS:000285538100011
PublisherHIGHER EDUCATION PRESS
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/69650
Collection中国科学院化学研究所
Corresponding AuthorChen Yi
Affiliation1.Chinese Acad Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China
2.Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Xie Ming-Yi,Guo Zhen-Peng,Chen Yi. Inspection into the Interaction of Bovine Serum Albumin with Gold Nanoparticles by Capillary Electrophoresis[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2010,31(11):2162-2166.
APA Xie Ming-Yi,Guo Zhen-Peng,&Chen Yi.(2010).Inspection into the Interaction of Bovine Serum Albumin with Gold Nanoparticles by Capillary Electrophoresis.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,31(11),2162-2166.
MLA Xie Ming-Yi,et al."Inspection into the Interaction of Bovine Serum Albumin with Gold Nanoparticles by Capillary Electrophoresis".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 31.11(2010):2162-2166.
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