ICCAS OpenIR
A direct continuous fluorometric turn-on assay for monoamine oxidase B and its inhibitor-screening based on the abnormal fluorescent behavior of silole
Peng, Lihua1,2; Zhang, Guanxin1; Zhang, Deqing1; Wang, Yilin1; Zhu, Daoben1
2010
Source PublicationANALYST
ISSN0003-2654
Volume135Issue:7Pages:1779-1784
AbstractAs essential enzymes in the body, monoamine oxidases (MAOs), including MAO-B, help maintain the proper balance of neurotransmitters and other dietary and biogenic amines. Thus, development of convenient and continuous assays for MAO-B and its inhibitor-screening is highly desirable since the inhibitors have implications for therapeutics for many brain diseases. In this paper, we report a direct continuous fluorescence turn-on assay for MAO-B and its inhibitor-screening with the ensemble of silole 1, heptylamine and HSO(3)(-), which are either easily accessible or commercially available. Fluorescent spectral investigations show that this fluorometric assay can be performed continuously in aqueous solution and MAO-B of concentrations as low as 0.25 mu g/mL can be analyzed. Moreover, the ensemble of silole 1, heptylamine, HSO(3)(-) and MAO-B can be employed to screen the inhibitors of MAO-B.
DOI10.1039/c0an00168f
Indexed BySCI
Language英语
WOS IDWOS:000279098000041
PublisherROYAL SOC CHEMISTRY
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/69600
Collection中国科学院化学研究所
Corresponding AuthorZhang, Guanxin
Affiliation1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Organ Solids Lab, Inst Chem, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Peng, Lihua,Zhang, Guanxin,Zhang, Deqing,et al. A direct continuous fluorometric turn-on assay for monoamine oxidase B and its inhibitor-screening based on the abnormal fluorescent behavior of silole[J]. ANALYST,2010,135(7):1779-1784.
APA Peng, Lihua,Zhang, Guanxin,Zhang, Deqing,Wang, Yilin,&Zhu, Daoben.(2010).A direct continuous fluorometric turn-on assay for monoamine oxidase B and its inhibitor-screening based on the abnormal fluorescent behavior of silole.ANALYST,135(7),1779-1784.
MLA Peng, Lihua,et al."A direct continuous fluorometric turn-on assay for monoamine oxidase B and its inhibitor-screening based on the abnormal fluorescent behavior of silole".ANALYST 135.7(2010):1779-1784.
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