ICCAS OpenIR
Rhodamine B Piperazinoacetohydrazine: A Water-Soluble Spectroscopic Reagent for Pyruvic Acid Labeling
Jia, Jia; Wang, Ke; Shi, Wen; Chen, Suming; Li, Xiaohua; Ma, Huimin
2010
Source PublicationCHEMISTRY-A EUROPEAN JOURNAL
ISSN0947-6539
Volume16Issue:22Pages:6638-6643
AbstractA new water-soluble reagent, rhodamine B piperazinoacetohydrazine (RBPH), with improved spectroscopic and reaction properties, has been developed and characterized for pyruvic acid labeling. The reagent RBPH is designed and synthesized by using rhodamine B as a spectroscopic unit, and hydrazine as a carbonyl-specific labeling unit: the two units are connected by a well-chosen linker of piperazine, which prohibits the formation of the non-fluorescent spirocyclic structure of rho-damine B, thereby keeping the spectroscopic response of the reagent in a stable state. Such a design enables RBPH not only to maintain its excellent spectroscopic properties over a wide pH range, but also to exist as a stable cation with high water solubility. Moreover, the hydrazino group of RBPH is expected to react selectively with carbonyl compounds under mild conditions through the rapid formation of hydrazones. These important features make RBPH of great potential use in the labeling of aldehydes or ketones in various biosystems, and such an application of RBPH as a precolumn derivatizing reagent has been successfully demonstrated on the analysis of pyruvic acid in human serum by high-performance liquid chromatography with common UV/Vis detection.
KeywordDerivatizing Reagents Fluorescence Pyruvic Acid Rhodamine b Uv/vis Spectroscopy
DOI10.1002/chem.200902660
Indexed BySCI ; IC
Language英语
WOS IDWOS:000279445400027
PublisherWILEY-BLACKWELL
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/69574
Collection中国科学院化学研究所
Corresponding AuthorMa, Huimin
AffiliationChinese Acad Sci, Inst Chem, Lab Life Analyt Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Jia, Jia,Wang, Ke,Shi, Wen,et al. Rhodamine B Piperazinoacetohydrazine: A Water-Soluble Spectroscopic Reagent for Pyruvic Acid Labeling[J]. CHEMISTRY-A EUROPEAN JOURNAL,2010,16(22):6638-6643.
APA Jia, Jia,Wang, Ke,Shi, Wen,Chen, Suming,Li, Xiaohua,&Ma, Huimin.(2010).Rhodamine B Piperazinoacetohydrazine: A Water-Soluble Spectroscopic Reagent for Pyruvic Acid Labeling.CHEMISTRY-A EUROPEAN JOURNAL,16(22),6638-6643.
MLA Jia, Jia,et al."Rhodamine B Piperazinoacetohydrazine: A Water-Soluble Spectroscopic Reagent for Pyruvic Acid Labeling".CHEMISTRY-A EUROPEAN JOURNAL 16.22(2010):6638-6643.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Jia, Jia]'s Articles
[Wang, Ke]'s Articles
[Shi, Wen]'s Articles
Baidu academic
Similar articles in Baidu academic
[Jia, Jia]'s Articles
[Wang, Ke]'s Articles
[Shi, Wen]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Jia, Jia]'s Articles
[Wang, Ke]'s Articles
[Shi, Wen]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.