ICCAS OpenIR
Recognition of Single- and Double-Stranded Oligonucleotides by Bovine Serum Albumin via Nonspecific Interactions
Xia, Yuqiong; Chen, Erqiang; Liang, Dehai1
2010-11-01
Source PublicationBIOMACROMOLECULES
ISSN1525-7797
Volume11Issue:11Pages:3158-3166
AbstractThe complexes formed by bovine serum albumin (BSA) with single-stranded oligonucleotide (ss-oligo) or double-stranded oligonucleotide (ds-oligo) were investigated by laser light scattering, zeta potential analysis, and atomic force microscopy. It was found that BSA was able to recognize ss-oligo and ds-oligo upon forming complexes in HCOOH-HCOONa buffer at pH 3.0. When oligonucleotide was added dropwisc to BSA. BSA formed a complex with ss-oligo but not with ds-oligo in the studied charge ratio. When BSA was added to oligonucleotides. BSA formed complexes with both ss-oligo and ds-oligo but via different paths: the BSA/ds-oligo underwent two processes, heavy precipitation followed by reentry, with increasing BSA/oligo charge ratio, whereas BSA/ss-oligo underwent only aggregation process. but with a charge reversal occurred at BSA/oligo charge ratio about 0.1. Moreover, the complex formed by BSA and ds-oligo showed a kinetics much slower than that of BSA and ss-oligo. We attributed the big difference upon complexation to the physical nature of oligonucleotides as well as the conformational change of BSA under severe conditions. The differentiation of ss-oligo from ds-oligo by BSA via nonspecific interactions gained insight in the recognition of DNA or RNA by specific protein (enzyme) under physiological conditions.
DOI10.1021/bm100969z
Indexed BySCI
Language英语
WOS IDWOS:000283810900043
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/69492
Collection中国科学院化学研究所
Corresponding AuthorLiang, Dehai
Affiliation1.Peking Univ, Coll Chem & Mol Engn, Minist Educ, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
2.Peking Univ, Coll Chem & Mol Engn, Minist Educ, Key Lab Polymer Chem & Phys, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Xia, Yuqiong,Chen, Erqiang,Liang, Dehai. Recognition of Single- and Double-Stranded Oligonucleotides by Bovine Serum Albumin via Nonspecific Interactions[J]. BIOMACROMOLECULES,2010,11(11):3158-3166.
APA Xia, Yuqiong,Chen, Erqiang,&Liang, Dehai.(2010).Recognition of Single- and Double-Stranded Oligonucleotides by Bovine Serum Albumin via Nonspecific Interactions.BIOMACROMOLECULES,11(11),3158-3166.
MLA Xia, Yuqiong,et al."Recognition of Single- and Double-Stranded Oligonucleotides by Bovine Serum Albumin via Nonspecific Interactions".BIOMACROMOLECULES 11.11(2010):3158-3166.
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