ICCAS OpenIR
A Water-Soluble Conjugated Polymer for Protein Identification and Denaturation Detection
Xu, Qingling; Wu, Chunxian; Zhu, Chunlei; Duan, Xinrui; Liu, Libing; Han, Yuchun; Wang, Yilin; Wang, Shu
2010
Source PublicationCHEMISTRY-AN ASIAN JOURNAL
ISSN1861-4728
Volume5Issue:12Pages:2524-2529
AbstractRapid and sensitive methods to detect proteins and protein denaturation have become increasingly needful in the field of protcomics, medical diagnostics, and biology. In this paper, we have reported the synthesis of a new cationic water-soluble conjugated polymer that contains fluorene and diene moieties in the backbone (PFDE) for protein identification by sensing an array of PFDE solutions in different ionic strengths using the linear discriminant analysis technique (LDA). The PFDE can form complexes with proteins by electrostatic and/or hydrophobic interactions and exhibits different fluorescence response. Three main factors contribute to the fluorescence response of PFDE, namely, the net charge density on the protein surface, the hydrophobic nature of the protein, and the metallo-protein characteristics. The denaturation of proteins can also be detected using PFDE as a fluorescent probe. The interactions between PFDE and proteins were also studied by dynamic light scattering (DLS) and isothermal titration microcalorimetry (ITC) techniques. In contrast to other methods based on conjugated polymers, the synthesis of a series of quencher or dye-labeled acceptors or protein substrates has been avoided in our method, which significantly reduces the cost and the synthetic complexity. Our method provides promising applications on protein identification and denaturation detection in a simple, fast, and label-free manner based on non-specific interaction-induced perturbation of PFDE fluorescence response.
KeywordConjugated Polymers Fluorescence Proteins Sensors
DOI10.1002/asia.201000363
Indexed BySCI
Language英语
WOS IDWOS:000285255600012
PublisherWILEY-V C H VERLAG GMBH
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/69452
Collection中国科学院化学研究所
Corresponding AuthorLiu, Libing
AffiliationChinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Xu, Qingling,Wu, Chunxian,Zhu, Chunlei,et al. A Water-Soluble Conjugated Polymer for Protein Identification and Denaturation Detection[J]. CHEMISTRY-AN ASIAN JOURNAL,2010,5(12):2524-2529.
APA Xu, Qingling.,Wu, Chunxian.,Zhu, Chunlei.,Duan, Xinrui.,Liu, Libing.,...&Wang, Shu.(2010).A Water-Soluble Conjugated Polymer for Protein Identification and Denaturation Detection.CHEMISTRY-AN ASIAN JOURNAL,5(12),2524-2529.
MLA Xu, Qingling,et al."A Water-Soluble Conjugated Polymer for Protein Identification and Denaturation Detection".CHEMISTRY-AN ASIAN JOURNAL 5.12(2010):2524-2529.
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