ICCAS OpenIR
Quantitative investigation of the influence of gold nanoparticles on the dynamics of DNA hybridization using a programmed multi-channel quartz crystal microbalance system
Li, Shaohua; Li, Xingchang; Zhang, Jianping; Zhang, Yakun; Han, Jianhua; Jiang, Long
2010-07-20
Source PublicationCOLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
ISSN0927-7757
Volume364Issue:1-3Pages:158-162
AbstractThis paper describes a quantitative investigation of the influence of gold nanoparticles (AuNPs) on DNA hybridization using a programmed multi-channel quartz crystal microbalance (QCM) device. This system has the benefit of continuous deposition, adsorption, washing, and drying of samples automatically, which saves time and enhances the accuracy of QCM measurements. A demonstration of the hybridization study has been carried out using a DNA fragment of the P53 gene near codon 248. The results demonstrated that the reliability and hybridization sensitivity of the QCM could be significantly improved by introducing AuNPs. The study revealed a significant increase in the apparent hybridization constant (K(D)(-1) of 5.29 x 10(10) M(-1)) with the addition of AuNPs, compared to those obtained without addition of AuNPs (about 10(7) M(-1)); this effect has not been reported before. The results of the experiment indicate the advantage of the multi-channel QCM chip in studying complicated DNA fixation and hybridization, the conjugating interaction between biomolecules and nanoparticles, and the hybridization dynamics. (C) 2010 Published by Elsevier B.V.
KeywordHybridization Quartz Crystal Microbalance Dna Transducer Gold Nanoparticles
DOI10.1016/j.colsurfa.2010.05.016
Indexed BySCI
Language英语
WOS IDWOS:000279833300025
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/69378
Collection中国科学院化学研究所
Corresponding AuthorJiang, Long
AffiliationChinese Acad Sci, Beijing Natl Lab Mol Sci BNLMS, Key Lab Colloid & Interface Sci, Inst Chem, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Li, Shaohua,Li, Xingchang,Zhang, Jianping,et al. Quantitative investigation of the influence of gold nanoparticles on the dynamics of DNA hybridization using a programmed multi-channel quartz crystal microbalance system[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2010,364(1-3):158-162.
APA Li, Shaohua,Li, Xingchang,Zhang, Jianping,Zhang, Yakun,Han, Jianhua,&Jiang, Long.(2010).Quantitative investigation of the influence of gold nanoparticles on the dynamics of DNA hybridization using a programmed multi-channel quartz crystal microbalance system.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,364(1-3),158-162.
MLA Li, Shaohua,et al."Quantitative investigation of the influence of gold nanoparticles on the dynamics of DNA hybridization using a programmed multi-channel quartz crystal microbalance system".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 364.1-3(2010):158-162.
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