ICCAS OpenIR
Transmembrane Delivery of Aggregated [Gd@C-82(OH)(22)](n) Nanoparticles
Zhang, Mingyi1,2; Xing, Gengmei1,2; Yuan, Hui1,2; Chang, Xueling1,2; Jing, Long1,2; Zhao, Yuliang1,2; Zhu, Chuanfeng3; Fang, Xiaohong3
2010-12-01
Source PublicationJOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
ISSN1533-4880
Volume10Issue:12Pages:8556-8561
AbstractWe investigated whether multi-hydroxyl metallofullerenes can penetrate into erythrocyte and whether this potential transmembrane delivery requires aggregated nanostructure of these particles. The metal atom encapsulated in metallofullerenes was used as a quantitative marker to investigate body distribution of aggregated nanoparticles in cytomembrane and cytoplasm. Image of atomic force microscopy (AFM) and assay of inductively coupled plasma-mass spectrometry (ICP-MS) suggested that aggregated 22 [Gd@C-82(OH)(22)](n) particles traversed through cytomembrane into belonged cytoplasm. Aggregated Gd nanostructure to small sphere with average diameter of, 22.4 +/- 0.5 nm. For pristine Gd@C-82 molecule, due to the electron donation from Gd atom, the distribution of electrons on the surface of carbon cage was localized. The electrophilic additive reaction of polyhydroxyl on the surface of Gd@C-82 was directly affected by local distribution of electrons. This resulted in local distribution of hydroxyls on the surface of Gd nanoparticles. Local distribution of hydroxyls brought about polar and nonpolar domains on particle surface, which induced Gd@C-82(OH)(22) to be amphiphilic molecule with due hydrophilic and hydrophobic properties. Amphiphilic properties of these molecules promoted their mutual aggregation in water. In the process of aggregation, amphiphilic properties of aggregated nanoparticles were well maintained, and besides, hydrophilic and hydrophobic domains were also regularly distributed on the surface of 22, [Gd@C-82(OH)(22)](n) particles. The amphiphilic nanoparticles attached externally to cytomembrane of, erythrocyte might be effectively driven by hydrophobic effect when they directly contacted cytomembrane of erythrocyte. The number of 22, [Gd@C-82(OH)(22)](n) nanoparticles attached to cytomembrane,n reached up to a certain crilical threshold, a significant curvature tension of membrane would occur. The shape of cell was accordingly changed. Increased membrane tension triggered the sudden opening of specific pores as a result of cytmennbrane response to nanoparitcle effect and the 22 [Gd@C-82(OH)(22)](n) nanoparticles gained entry to cell via these pores. This process was biologically independent of caveolar-mediated endocytosis or transportation pathway via ion channels.
KeywordTransmembrane Delivery Gd@c-82(Oh)(22) Erythrocyte
DOI10.1166/jnn.2010.2490
Indexed BySCI ; ISTP
Language英语
WOS IDWOS:000283621400093
PublisherAMER SCIENTIFIC PUBLISHERS
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/69372
Collection中国科学院化学研究所
Corresponding AuthorXing, Gengmei
Affiliation1.Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China
2.Natl Ctr Nanosci & Technol China, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Mingyi,Xing, Gengmei,Yuan, Hui,et al. Transmembrane Delivery of Aggregated [Gd@C-82(OH)(22)](n) Nanoparticles[J]. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2010,10(12):8556-8561.
APA Zhang, Mingyi.,Xing, Gengmei.,Yuan, Hui.,Chang, Xueling.,Jing, Long.,...&Fang, Xiaohong.(2010).Transmembrane Delivery of Aggregated [Gd@C-82(OH)(22)](n) Nanoparticles.JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,10(12),8556-8561.
MLA Zhang, Mingyi,et al."Transmembrane Delivery of Aggregated [Gd@C-82(OH)(22)](n) Nanoparticles".JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 10.12(2010):8556-8561.
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