ICCAS OpenIR
Universal Platform for Sensitive and Label-Free Nuclease Assay Based on Conjugated Polymer and DNA/Intercalating Dye Complex
Pu, Fang1; Hu, Dan1; Ren, Jinsong1; Wang, Shu2; Qu, Xiaogang1
2010-03-16
Source PublicationLANGMUIR
ISSN0743-7463
Volume26Issue:6Pages:4540-4545
AbstractBy taking advantage of the large signal amplification through efficient fluorescence resonance energy transfer (FRET) from the conjugated polymer to the intercalating dye mediated by DNA, a new strategy for nuclease assay has been developed using conjugated polymer and DNA/intercalating dye complex. The discrimination of DNA before or after digestion by nuclease denotes the universal application of the approach, in which either dsDNA or ssDNA substrates could be used for detecting nuclease activity. This method can be extended to most nucleases by simply changing the Substrate DNA. The present method is label-free, rapid,and highly sensitive, with a detection limit Much better or at least comparable to previous reports. fit addition, this assay is easy to implement for Visual detection with the assistance of a UV transilluminator. We reason that a similar strategy can be adopted for the detection of other analytes.
DOI10.1021/la904173j
Indexed BySCI
Language英语
WOS IDWOS:000275226700111
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:48[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/69346
Collection中国科学院化学研究所
Corresponding AuthorRen, Jinsong
Affiliation1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Rare Earth Resources Utilizat, Changchun 130022, Peoples R China
2.Chinese Acad Sci, Key Lab Organ Solids, Inst Chem, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Pu, Fang,Hu, Dan,Ren, Jinsong,et al. Universal Platform for Sensitive and Label-Free Nuclease Assay Based on Conjugated Polymer and DNA/Intercalating Dye Complex[J]. LANGMUIR,2010,26(6):4540-4545.
APA Pu, Fang,Hu, Dan,Ren, Jinsong,Wang, Shu,&Qu, Xiaogang.(2010).Universal Platform for Sensitive and Label-Free Nuclease Assay Based on Conjugated Polymer and DNA/Intercalating Dye Complex.LANGMUIR,26(6),4540-4545.
MLA Pu, Fang,et al."Universal Platform for Sensitive and Label-Free Nuclease Assay Based on Conjugated Polymer and DNA/Intercalating Dye Complex".LANGMUIR 26.6(2010):4540-4545.
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