ICCAS OpenIR
Class-specific immunoaffinity monolith for efficient on-line clean-up of pyrethroids followed by high-performance liquid chromatography analysis
Liang, Yuan; Zhou, Shuang; Hu, Liming; Li, Lin; Zhao, Meiping; Liu, Huwei
2010-01-15
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
Volume878Issue:2Pages:278-282
AbstractA class-specific monolithic immunoaffinity column was developed for on-line clean-up of pyrethroid insecticides in complex samples Deltamethrin was oxidized with ozone to generate the hapten of (RS)-acyano-alpha-phenoxybenzyl (RS)-cis,traps-2,2-dimethyl-3-formyl-cyclopropane carboxylate Class-specific antibodies against pyrethroids were produced using the conjugate of above hapten with bovine serum albumin as the immunogen. Poly(ethylene dimethacrylate-glycidyl methacrylate) monolith was synthesized in a 50 mm, 4.6 mm id stainless steel cartridge with two auxiliary pipette tips. The polymerization method was proved to be economic and reproducible. Antibodies against pyrethrotds were covalently immobilized onto the monolithic support via Schiff base reaction With a column-switching valve system, the immunoaffinity monolith (IAM) could be readily adapted to the reversed-phase high-performance liquid chromatography (HPLC) system. Under the optimum loading, washing and eluting conditions, the IAM specifically retained deltamethrin, flumethrin. flucythrinate and cis/traps permethrin, which were further baseline separated by C18 column using acetonitrile-water (83:17, V/V) as the mobile phase at a flow rate of 1.0 mL(min. The established system provides a highly efficient approach for high-throughput on-line clean-up of pyrethroid in various samples. (C) 2009 Elsevier B.V. All rights reserved.
KeywordImmunoaffinity Monolith Class-specific Pyrethroid On-line Clean-up
DOI10.1016/j.jchromb.2009.06.007
Indexed BySCI
Language英语
WOS IDWOS:000274220900022
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/69274
Collection中国科学院化学研究所
Corresponding AuthorZhao, Meiping
AffiliationPeking Univ, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn, BNLMS,Minist Educ, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Liang, Yuan,Zhou, Shuang,Hu, Liming,et al. Class-specific immunoaffinity monolith for efficient on-line clean-up of pyrethroids followed by high-performance liquid chromatography analysis[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2010,878(2):278-282.
APA Liang, Yuan,Zhou, Shuang,Hu, Liming,Li, Lin,Zhao, Meiping,&Liu, Huwei.(2010).Class-specific immunoaffinity monolith for efficient on-line clean-up of pyrethroids followed by high-performance liquid chromatography analysis.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,878(2),278-282.
MLA Liang, Yuan,et al."Class-specific immunoaffinity monolith for efficient on-line clean-up of pyrethroids followed by high-performance liquid chromatography analysis".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 878.2(2010):278-282.
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