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Adsorptive Immobilization of Four Proteases on Different Molecular Sieves
Liu Ping; Xing Guo-Wen; Li Xuan-Wen; Ye Yun-Hua
2010-04-01
Source PublicationACTA PHYSICO-CHIMICA SINICA
ISSN1000-6818
Volume26Issue:4Pages:1113-1118
AbstractThe immobilization of four different proteases on a series of molecular sieves, including microporous HY, NaY, NH4Y, MCM-22, H beta zeolites, modified Y zeolites HDAY, HNH(4)DAY, and mesoporous MCM-41, by adsorption was systematically investigated The four proteases were alpha-chymotrypsin, papain, subtilisin, and thermoase (or its pure form thermolysin) The results showed that the enzyme loading amount and the activity of immobilized enzyme were significantly affected not only by the structures and textures of molecular sieves and the enzyme properties. but also by the adsorptive conditions such as buffer pH values and enzyme concentration In most cases, the amount of protease loading on molecular sieves was relatively higher at pH 6 0, but declined with further increasing of pH values The nature of the interaction between protease and molecular sieves is discussed As for alpha-chymotrypsin, its loading amount on H beta zeolites was found to be the highest, whereas the activity of a-chymotrypsin immobilized on MCM-22 was the highest, which is probably due to different adsorption states.
KeywordAdsorption Immobilization Protease Zeolite Molecular Sieve Peptide Synthesis
DOI10.3866/PKU.WHXB20100448
Indexed BySCI
Language英语
WOS IDWOS:000276958400041
PublisherPEKING UNIV PRESS
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/69064
Collection中国科学院化学研究所
Corresponding AuthorYe Yun-Hua
AffiliationPeking Univ, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci,Minist Educ, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Liu Ping,Xing Guo-Wen,Li Xuan-Wen,et al. Adsorptive Immobilization of Four Proteases on Different Molecular Sieves[J]. ACTA PHYSICO-CHIMICA SINICA,2010,26(4):1113-1118.
APA Liu Ping,Xing Guo-Wen,Li Xuan-Wen,&Ye Yun-Hua.(2010).Adsorptive Immobilization of Four Proteases on Different Molecular Sieves.ACTA PHYSICO-CHIMICA SINICA,26(4),1113-1118.
MLA Liu Ping,et al."Adsorptive Immobilization of Four Proteases on Different Molecular Sieves".ACTA PHYSICO-CHIMICA SINICA 26.4(2010):1113-1118.
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