ICCAS OpenIR
High-light induced singlet oxygen formation in cytochrome b(6)f complex from Bryopsis corticulans as detected by EPR spectroscopy
Sang, Min1,5; Ma, Fei2; Xie, Jie3; Chen, Xiao-Bo4; Wang, Ke-Bin1; Qin, Xiao-Chun1; Wang, Wen-Da1; Zhao, Jing-Quan3; Li, Liang-Bi1; Zhang, Jian-Ping2; Kuang, Ting-Yun1
2010
Source PublicationBIOPHYSICAL CHEMISTRY
ISSN0301-4622
Volume146Issue:1Pages:7-12
AbstractElectron paramagnetic resonance (EPR) spectroscopy was used to detect the light-induced formation of singlet oxygen (O-1(2)center dot) in the intact and the Rieske-depleted cytochrome b(6)f complexes (Cyt b(6)f) from Bryopsis corticulans, as well as in the isolated Rieske Fe-S protein. It is shown that, under white-light illumination and aerobic conditions, chlorophyll a (Chl a) bound in the intact Cyt b(6)f can be bleached by light-induced O-1(2)center dot, and that the 10; production can be promoted by D2O or scavenged by extraneous antioxidants such as L-histidine, ascorbate, beta-carotene and glutathione. Under similar experimental conditions, O-1(2)center dot was also detected in the Rieske-depleted Cyt b(6)f complex, but not in the isolated Rieske Fe-S protein. The results prove that Chl a cofactor, rather than Rieske Fe-S protein, is the specific site of O-1(2)center dot formation, a conclusion which draws further support from the generation of O-1(2)center dot with selective excitation of Chl a using monocolor red light. (C) 2009 Elsevier B.V. All rights reserved.
KeywordChlorophyll a Cyt b(6)f Singlet Oxygen Photo-bleaching Epr
DOI10.1016/j.bpc.2009.09.012
Indexed BySCI
Language英语
WOS IDWOS:000272440400002
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/69052
Collection中国科学院化学研究所
Corresponding AuthorLi, Liang-Bi
Affiliation1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China
2.Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China
3.Chinese Acad Sci, Inst Chem, Key Lab Photochem, Beijing 100080, Peoples R China
4.Hebei Univ Sci & Technol, Sch Biosci & Engn, Shijiazhuang 050018, Hebei Province, Peoples R China
5.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Sang, Min,Ma, Fei,Xie, Jie,et al. High-light induced singlet oxygen formation in cytochrome b(6)f complex from Bryopsis corticulans as detected by EPR spectroscopy[J]. BIOPHYSICAL CHEMISTRY,2010,146(1):7-12.
APA Sang, Min.,Ma, Fei.,Xie, Jie.,Chen, Xiao-Bo.,Wang, Ke-Bin.,...&Kuang, Ting-Yun.(2010).High-light induced singlet oxygen formation in cytochrome b(6)f complex from Bryopsis corticulans as detected by EPR spectroscopy.BIOPHYSICAL CHEMISTRY,146(1),7-12.
MLA Sang, Min,et al."High-light induced singlet oxygen formation in cytochrome b(6)f complex from Bryopsis corticulans as detected by EPR spectroscopy".BIOPHYSICAL CHEMISTRY 146.1(2010):7-12.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Sang, Min]'s Articles
[Ma, Fei]'s Articles
[Xie, Jie]'s Articles
Baidu academic
Similar articles in Baidu academic
[Sang, Min]'s Articles
[Ma, Fei]'s Articles
[Xie, Jie]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Sang, Min]'s Articles
[Ma, Fei]'s Articles
[Xie, Jie]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.