ICCAS OpenIR
Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles
Wang, Xinyi2; Xu, Yao1; Xu, Xiao1; Hu, Ke1; Xiang, Minghui1; Li, Limei2; Liu, Feng1; Li, Na1
2010-07-15
Source PublicationTALANTA
ISSN0039-9140
Volume82Issue:2Pages:693-697
AbstractThe purpose of this study was to establish a simple and sensitive analytical method for lysozyme using Plasmon Resonance Light-Scattering (PRLS) technique with Gold Nanoparticles (AuNPs) as the probe. Nanomolar level of lysozyme induced AuNPs aggregation with enhanced PRLS. For 1.4 nM citrate-capped AuNPs (13 nm in diameter), the linear range of the calibration curve was 15-50 nM with a detection limit of 13.1 nM for lysozyme. Six nanomolar lysozyme can produce an observable PRLS enhancement. Most potential interfering substances present in urine had a negligible effect on the determination. The interference from human serum albumin in the urinary sample can be reduced by precipitating the albumin with ethanol at pH 4.8-4.9. The 90.1-118.2% recovery was achieved for 8 individual lysozyme-spiked urinary samples. This simple and sensitive method for lysozyme does not require sample clean-up and AuNPs modification, thus provided an alternative for urinary lysozyme determination. (C) 2010 Elsevier B.V. All rights reserved.
KeywordLysozyme Plasmon Resonance Light-scattering Gold Nanoparticles Urinary Sample
DOI10.1016/j.talanta.2010.05.034
Indexed BySCI
Language英语
WOS IDWOS:000280375700040
PublisherELSEVIER SCIENCE BV
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Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/69000
Collection中国科学院化学研究所
Corresponding AuthorLi, Na
Affiliation1.Peking Univ, BNLMS, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Coll Chem & Mol Engn, Beijing 100871, Peoples R China
2.Shenyang Agr Univ, Coll Sci, Shenyang 110161, Peoples R China
Recommended Citation
GB/T 7714
Wang, Xinyi,Xu, Yao,Xu, Xiao,et al. Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles[J]. TALANTA,2010,82(2):693-697.
APA Wang, Xinyi.,Xu, Yao.,Xu, Xiao.,Hu, Ke.,Xiang, Minghui.,...&Li, Na.(2010).Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles.TALANTA,82(2),693-697.
MLA Wang, Xinyi,et al."Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles".TALANTA 82.2(2010):693-697.
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