ICCAS OpenIR
Noncovalent Attachment of NAD(+) Cofactor onto Carbon Nanotubes for Preparation of Integrated Dehydrogenase-Based Electrochemical Biosensors
Zhou, Haojie1; Zhang, Zipin1; Yu, Ping1; Su, Lei1; Ohsaka, Takeo2; Mao, Lanqun1
2010-04-20
Source PublicationLANGMUIR
ISSN0743-7463
Volume26Issue:8Pages:6028-6032
AbstractThis study describes a facile approach to the preparation of integrated dehydrogenase-based electrochemical biosensors through noncovalent attachment (Tan oxidized form of beta-nicotinamide adenine dinucleotide (NAD(+)) onto carbon nanotubes with the interaction between the adenine subunit in NAD(+) molecules and multiwalled carbon nanotubes (MWCNTs). X-ray photoelectron spectroscopic and cyclic voltammetric results suggest that NAD(+) is noncovalently attached onto MWCNTs to form an NAD(+)/MWCNT composite that acts as the electronic transducer for the integrated dehydrogenase-based electrochemical biosensors. With glucose dehydrogenase (GDH) as a model dehydrogenase-based recognition unit, electrochemical studies reveal that glucose is readily oxidized at the GDH/NAD(+)/MWCNT-modified electrode without addition of NAD(+) in the phosphate buffer. The potential for the oxidation of glucose at the GDH/NAD(+)/MWCNT-modified electrode remains very close to that for NADH oxidation at the MWCNT-modified electrode, but it is more negative than those for the oxidation of glucose at the MWCNT-modified electrode and for NADH oxidation at a bare glassy carbon electrode. These results demonstrate that NAD(+) molecules stably attached onto MWCNTs efficiently act as the cofactor for the dehydrogenases. MWCNTs employed here not only serve as the electronic transducer and the support to confine NAD(+) cofactor onto the electrode surface, hut also act as the electrocatalyst For NADH oxidation in the dehydrogenase-based electrochemical biosensors. At the GDH/NAD(+)/MWCNT-based glucose biosensor, the current is linear with the concentration of glucose being within a concentration ranee from 10 to 300 mu M with a limit of detection down to 4.81 mu M (S/N = 3). This study offers a facile and versatile approach to the development of integrated dehydrogenase-based electrochemical devices, such as electrochemical biosensors and biofuel cells.
DOI10.1021/la903799n
Indexed BySCI
Language英语
WOS IDWOS:000276562300098
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:48[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/68988
Collection中国科学院化学研究所
Corresponding AuthorMao, Lanqun
Affiliation1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Living Biosyst, Inst Chem, Beijing 100190, Peoples R China
2.Tokyo Inst Technol, Dept Elect Chem, Interdisciplinary Grad Sch Sci & Engn, Midori Ku, Yokohama, Kanagawa 2268502, Japan
Recommended Citation
GB/T 7714
Zhou, Haojie,Zhang, Zipin,Yu, Ping,et al. Noncovalent Attachment of NAD(+) Cofactor onto Carbon Nanotubes for Preparation of Integrated Dehydrogenase-Based Electrochemical Biosensors[J]. LANGMUIR,2010,26(8):6028-6032.
APA Zhou, Haojie,Zhang, Zipin,Yu, Ping,Su, Lei,Ohsaka, Takeo,&Mao, Lanqun.(2010).Noncovalent Attachment of NAD(+) Cofactor onto Carbon Nanotubes for Preparation of Integrated Dehydrogenase-Based Electrochemical Biosensors.LANGMUIR,26(8),6028-6032.
MLA Zhou, Haojie,et al."Noncovalent Attachment of NAD(+) Cofactor onto Carbon Nanotubes for Preparation of Integrated Dehydrogenase-Based Electrochemical Biosensors".LANGMUIR 26.8(2010):6028-6032.
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