ICCAS OpenIR
Polymer mixtures with enhanced compatibility and extremely low viscosity used as DNA separation media
Ke, Fuyou1; Mo, Xiulei1; Yang, Runmiao2; Wang, Yanmei2; Liang, Dehai1
2010-02-01
Source PublicationELECTROPHORESIS
ISSN0173-0835
Volume31Issue:3Pages:520-527
AbstractPoor compatibility was the major drawback of polymer mixtures when used as DNA separation media. Using poly(ethylene oxide)-b-poly(N, N-dimethylacrylamide) (PEO(44)-b-PDMA(88)) and PEO (M(w): 1.3 MDa) as an example, we demonstrated the concept that the compatibility was significantly improved when mixing a homopolymer with its copolymer. Laser light scattering indicated that the major interaction between PEO and PEO-b-PDMA in dilute solution was the weak hydrodynamic interaction, which showed almost no effect on the viscosity and the static scattering pattern. In semidilute or concentrated solution, viscosity measurement also suggested good compatibility between the two components. When tested as DNA separation medium by CE, the viscosity of the mixture was extremely low, only 5 cP for 5.0 m/v% PEO-b-PDMA+0.1 m/v% PEO at 25 degrees C. The performance on DNA separation could be tuned by varying the concentration of each component as well as the component ratio. Good separation on both dsDNA and ssDNA was achieved.
KeywordDna Separation Electrophoresis Laser Light Scattering Polymer Mixtures Viscometry
DOI10.1002/elps.200900440
Indexed BySCI
Language英语
WOS IDWOS:000274603200011
PublisherWILEY-V C H VERLAG GMBH
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/68882
Collection中国科学院化学研究所
Corresponding AuthorLiang, Dehai
Affiliation1.Peking Univ, Coll Chem & Mol Engn, Minist Educ, Key Lab Polymer Chem & Phys,Beijing Natl Lab Mol, Beijing 100871, Peoples R China
2.Univ Sci & Technol China, Dept Polymer Sci & Engn, Hefei 230026, Peoples R China
Recommended Citation
GB/T 7714
Ke, Fuyou,Mo, Xiulei,Yang, Runmiao,et al. Polymer mixtures with enhanced compatibility and extremely low viscosity used as DNA separation media[J]. ELECTROPHORESIS,2010,31(3):520-527.
APA Ke, Fuyou,Mo, Xiulei,Yang, Runmiao,Wang, Yanmei,&Liang, Dehai.(2010).Polymer mixtures with enhanced compatibility and extremely low viscosity used as DNA separation media.ELECTROPHORESIS,31(3),520-527.
MLA Ke, Fuyou,et al."Polymer mixtures with enhanced compatibility and extremely low viscosity used as DNA separation media".ELECTROPHORESIS 31.3(2010):520-527.
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