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A non-oxidative electrochemical approach to online measurements of dopamine release through laccase-catalyzed oxidation and intramolecular cyclization of dopamine
Lin, Yuqing; Zhang, Zipin; Zhao, Lingzhi; Wang, Xiang; Yu, Ping; Su, Lei; Mao, Lanqun
2010-02-15
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
Volume25Issue:6Pages:1350-1355
AbstractA new electrochemical approach to selective online measurements of dopamine (DA) release in the cerebral microdialysate is demonstrated with a non-oxidative mechanism based on the distinct reaction properties of DA and the excellent biocatalytic activity of laccase. To make the successful transition of the distinct sequential reaction properties of DA from a conceptual determination protocol to a practical online analytical system, laccase enzyme is immobilized onto magnetite nanoparticles and the nanoparticles are confined into a fused-silica capillary through an external magnetic field to fabricate a magnetic microreactor. The microreactor is placed in the upstream of the thin-layer electrochemical flow cell to efficiently catalyze the oxidation of DA into its quinonoid form and thereby initialize the sequential reactions including deprotonation, intramolecular cyclization, disproportionation and/or oxidation to finally give 5,6-dihydroxyindoline quinone. The electrochemical reduction of the produced 5,6-dihydroxyindoline quinone at bare glassy carbon electrode is used as the readout for the DA measurement. The laccase-immobilized microreactor is also found to catalyze the oxidation of ascorbic acid (AA) and 3,4-dihydroxyphenylacetic acid (DOPAC) into electroinactive species and, as such, to eliminate the great interference from both species. Moreover, the successful transition of the mechanism for DA detection from the conventional oxidative electrochemical approach to the non-oxidative one substantially enables the measurements virtually interference-free from physiological levels of uric acid, 5-hydroxytryptamine, norepinephrine, and epinephrine. The current response is linear with DA concentration within a concentration range from 1 to 20 mu M with a sensitivity of 3.97 nA/mu M. The detection limit, based on a signal-to-noise ratio of 3, is calculated to be 0.3 mu M. The high selectivity and the good linearity as well as the high stability of the online method make it very potential for continuous monitoring of cerebral DA release in physiological and pathological processes. (C) 2009 Elsevier B.V. All rights reserved.
KeywordOnline Electrochemical Analysis Enzyme Microreactor Dopamine Laccase
DOI10.1016/j.bios.2009.10.028
Indexed BySCI
Language英语
WOS IDWOS:000274868800016
PublisherELSEVIER ADVANCED TECHNOLOGY
Citation statistics
Cited Times:37[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/68878
Collection中国科学院化学研究所
Corresponding AuthorMao, Lanqun
AffiliationChinese Acad Sci, Inst Chem, Lab Life Analyt Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Lin, Yuqing,Zhang, Zipin,Zhao, Lingzhi,et al. A non-oxidative electrochemical approach to online measurements of dopamine release through laccase-catalyzed oxidation and intramolecular cyclization of dopamine[J]. BIOSENSORS & BIOELECTRONICS,2010,25(6):1350-1355.
APA Lin, Yuqing.,Zhang, Zipin.,Zhao, Lingzhi.,Wang, Xiang.,Yu, Ping.,...&Mao, Lanqun.(2010).A non-oxidative electrochemical approach to online measurements of dopamine release through laccase-catalyzed oxidation and intramolecular cyclization of dopamine.BIOSENSORS & BIOELECTRONICS,25(6),1350-1355.
MLA Lin, Yuqing,et al."A non-oxidative electrochemical approach to online measurements of dopamine release through laccase-catalyzed oxidation and intramolecular cyclization of dopamine".BIOSENSORS & BIOELECTRONICS 25.6(2010):1350-1355.
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