ICCAS OpenIR
Characterizing DNA Condensation and Conformational Changes in Organic Solvents
Ke, Fuyou1,2; Luu, Yen Kim3; Hadjiargyrou, Michael3; Liang, Dehai1,2
2010-10-11
Source PublicationPLOS ONE
ISSN1932-6203
Volume5Issue:10
AbstractOrganic solvents offer a new approach to formulate DNA into novel structures suitable for gene delivery. In this study, we examined the in situ behavior of DNA in N, N-dimethylformamide (DMF) at low concentration via laser light scattering (LLS), TEM, UV absorbance and Zeta potential analysis. Results revealed that, in DMF, a 21bp oligonucleotide remained intact, while calf thymus DNA and supercoiled plasmid DNA were condensed and denatured. During condensation and denaturation, the size was decreased by a factor of 8-10, with calf thymus DNA forming spherical globules while plasmid DNA exhibited a toroid-like conformation. In the condensed state, DNA molecules were still able to release the counterions to be negatively charged, indicating that the condensation was mainly driven by the excluded volume interactions. The condensation induced by DMF was reversible for plasmid DNA but not for calf thymus DNA. When plasmid DNA was removed from DMF and resuspended in an aqueous solution, the DNA was quickly regained a double stranded configuration. These findings provide further insight into the behavior and condensation mechanism of DNA in an organic solvent and may aid in developing more efficient non-viral gene delivery systems.
DOI10.1371/journal.pone.0013308
Indexed BySCI
Language英语
WOS IDWOS:000282748100030
PublisherPUBLIC LIBRARY SCIENCE
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/68710
Collection中国科学院化学研究所
Corresponding AuthorKe, Fuyou
Affiliation1.Peking Univ, Coll Chem & Mol Engn, Minist Educ, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
2.Peking Univ, Coll Chem & Mol Engn, Minist Educ, Key Lab Polymer Chem & Phys, Beijing 100871, Peoples R China
3.SUNY Stony Brook, Dept Biomed Engn, Stony Brook, NY 11794 USA
Recommended Citation
GB/T 7714
Ke, Fuyou,Luu, Yen Kim,Hadjiargyrou, Michael,et al. Characterizing DNA Condensation and Conformational Changes in Organic Solvents[J]. PLOS ONE,2010,5(10).
APA Ke, Fuyou,Luu, Yen Kim,Hadjiargyrou, Michael,&Liang, Dehai.(2010).Characterizing DNA Condensation and Conformational Changes in Organic Solvents.PLOS ONE,5(10).
MLA Ke, Fuyou,et al."Characterizing DNA Condensation and Conformational Changes in Organic Solvents".PLOS ONE 5.10(2010).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ke, Fuyou]'s Articles
[Luu, Yen Kim]'s Articles
[Hadjiargyrou, Michael]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ke, Fuyou]'s Articles
[Luu, Yen Kim]'s Articles
[Hadjiargyrou, Michael]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ke, Fuyou]'s Articles
[Luu, Yen Kim]'s Articles
[Hadjiargyrou, Michael]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.