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A chiral ligand exchange CE essay with zinc(II)-L-valine complex for determining enzyme kinetic constant of L-amino acid oxidase
Qi, Li1,2,4; Yang, Gengliang4; Zhang, Haizhi1,2,3; Qiao, Juan1,2,3
2010-06-15
Source PublicationTALANTA
ISSN0039-9140
Volume81Issue:4-5Pages:1554-1559
AbstractA new strategy for the enantioseparation of D,L-amino acids employing the principle of ligand exchange capillary electrophoresis with Zn(II)-L-valine complex as a chiral selecting system in the presence of beta-cyclodextrin has been designed. Successful enantioseparation of label flee and labeled amino acids have been achieved with a buffer of 100 0 mM boric acid, 5 0 mM ammonium acetate, 4.0 mM beta-cyclodextrin, 4 0 mM ZnSO(4) and 8 0 mM L-valine at pH 8.1 This new method was shown to be applicable to the quantitative analysis of label free D- and L-aromatic amino acids Furthermore, the expanding enzymatic use of L-amino acid oxidase to incubate with different L-amino acids has allowed understanding of the substrate's specificity. An on-column incubation assay has been developed to study the L-amino acid oxidase's catalytic efficiency. It was demonstrated that the enzyme kinetic constant could be determined by using this new method (C) 2010 Elsevier B V. All rights reserved.
KeywordEnzyme Kinetics L-amino Acid Oxidase Ligand Exchange Ce Zinc Complex Enantioseparation
DOI10.1016/j.talanta.2010.03.001
Indexed BySCI
Language英语
WOS IDWOS:000278737100062
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:28[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/68690
Collection中国科学院化学研究所
Corresponding AuthorQi, Li
Affiliation1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
4.Hebei Univ, Coll Pharm, Baoding 071002, Peoples R China
Recommended Citation
GB/T 7714
Qi, Li,Yang, Gengliang,Zhang, Haizhi,et al. A chiral ligand exchange CE essay with zinc(II)-L-valine complex for determining enzyme kinetic constant of L-amino acid oxidase[J]. TALANTA,2010,81(4-5):1554-1559.
APA Qi, Li,Yang, Gengliang,Zhang, Haizhi,&Qiao, Juan.(2010).A chiral ligand exchange CE essay with zinc(II)-L-valine complex for determining enzyme kinetic constant of L-amino acid oxidase.TALANTA,81(4-5),1554-1559.
MLA Qi, Li,et al."A chiral ligand exchange CE essay with zinc(II)-L-valine complex for determining enzyme kinetic constant of L-amino acid oxidase".TALANTA 81.4-5(2010):1554-1559.
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