ICCAS OpenIR
The bioactivity of rhBMP-2 immobilized poly(lactide-co-glycolide) scaffolds
Shen, Hong; Hu, Xixue; Yang, Fei; Bei, Jianzhong; Wang, Shenguo
2009-06-01
Source PublicationBIOMATERIALS
ISSN0142-9612
Volume30Issue:18Pages:3150-3157
AbstractIn this study, immobilization of rhBMP-2 on polylactone-type polymer scaffolds via plasma treatment was investigated. To introduce proper functional groups on the surface of poly(lactide-co-glycolide) (PLGA) matrix, PLGA films were treated under different atmospheres, such as oxygen, ammonia and carbon dioxide, respectively, and then incubated in rhBMP-2 solution of de-ionized water. The effect of various plasma-treated PLGA films on binding rhBMP-2 was investigated and compared. It was found that PLGA binding ability to rhBMP-2 was enhanced by carbon dioxide and oxygen plasma treatment, and the binding ability of the oxygen plasma-treated PLGA (OT-PLGA) to rhBMP-2 was the strongest after oxygen plasma treating for 10 min under a power of 50 W. The changes of surface chemistry and surface topography of PLGA matrix induced by oxygen plasma treatment played main roles in improving the PLGA binding ability to rhBMP-2. The stability of rhBMP-2 bound on OT-PLGA film was determined under a dynamic condition by a Parallel Plate Flow Chamber. The result showed that the rhBMP-2 had been immobilized on the OT-PLGA film. Mouse OCT-1 osteoblast-like cell as a model cell was cultured on the rhBMP-2 bound OT-PLGA (OT-PLGA/BMP) in vitro, which showed that the bound rhBMP-2 via oxygen plasma treatment was bioactive. Depending on hydrophilicity and rich polar O-containing groups of the OT-PLGA scaffold, different amount of rhBMP-2 could be evenly immobilized on the surface of the OT-PLGA scaffold. The immobilized rhBMP-2 had stimulated differentiation of OCT-1 cell and accelerated process of mineralization of OCT-1 cell in the scaffold. It revealed the rhBMP-2 immobilized PLGA scaffold had good cell affinity. (C) 2009 Elsevier Ltd. All rights reserved.
KeywordRhbmp-2 Oxygen Plasma Treatment Anchorage Immobilization Plga Scaffold Tissue Engineering
DOI10.1016/j.biomaterials.2009.02.004
Indexed BySCI
Language英语
WOS IDWOS:000266148200004
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:61[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/68624
Collection中国科学院化学研究所
Corresponding AuthorWang, Shenguo
AffiliationChinese Acad Sci, Inst Chem, State Key Lab Polymer Phys & Chem, BNLMS, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Shen, Hong,Hu, Xixue,Yang, Fei,et al. The bioactivity of rhBMP-2 immobilized poly(lactide-co-glycolide) scaffolds[J]. BIOMATERIALS,2009,30(18):3150-3157.
APA Shen, Hong,Hu, Xixue,Yang, Fei,Bei, Jianzhong,&Wang, Shenguo.(2009).The bioactivity of rhBMP-2 immobilized poly(lactide-co-glycolide) scaffolds.BIOMATERIALS,30(18),3150-3157.
MLA Shen, Hong,et al."The bioactivity of rhBMP-2 immobilized poly(lactide-co-glycolide) scaffolds".BIOMATERIALS 30.18(2009):3150-3157.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Shen, Hong]'s Articles
[Hu, Xixue]'s Articles
[Yang, Fei]'s Articles
Baidu academic
Similar articles in Baidu academic
[Shen, Hong]'s Articles
[Hu, Xixue]'s Articles
[Yang, Fei]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Shen, Hong]'s Articles
[Hu, Xixue]'s Articles
[Yang, Fei]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.