ICCAS OpenIR
Imaging Biocatalytic Activity of Enzyme-Polymer Spots by Means of Combined Scanning Electrochemical Microscopy/Electrogenerated Chemiluminescence
Lei, Rong1,2; Stratmann, Lutz2; Schaefer, Dominik2; Erichsen, Thomas2; Neugebauer, Sebastian2; Li, Na1; Schuhmann, Wolfgang2
2009-06-15
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume81Issue:12Pages:5070-5074
AbstractThe purpose of this study was to develop a scanning electrochemical microscopy (SECM) and scanning electrogenerated chemiluminescence (SECL) setup to visualize the localized enzymatic activity using glucose oxidase as a model. Combination of SECM and electrogenerated chemiluminescence (ECL) was made possible by integrating a photomultiplier tube (PMT) within a SECM setup which is mounted on top of an inverted microscope. An enzyme-polymer spot formed on a glass slide and placed on top of the entrance window of the PMT was used as a model sample to evaluate the potential of the combined SECM/ECL setup. Hydrogen peroxide, which was locally generated by the glucose oxidase (GOx)-catalyzed reaction, reacted with oxidized luminol which was simultaneously electrochemically generated at the positioned SECM electrode tip. By using the phase-sensitive lock-in amplifier, the potential applied to the SECM tip was sinusoidally swept to invoke an associated oscillation of the ECL. Thus, sensitivity of SECL could be substantially enhanced. Images of the local immobilized enzyme activity obtained both by ECL and generator/collector (GC) mode of SECM were compared to elucidate the pathway in which the SECM and SECL signals are generated.
DOI10.1021/ac900192n
Indexed BySCI
Language英语
WOS IDWOS:000266969700051
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/68580
Collection中国科学院化学研究所
Corresponding AuthorLi, Na
Affiliation1.Peking Univ, Coll Chem & Mol Engn, Inst Analyt Chem, BNLMS,Minist Educ,Key Lab Bioorgan Chem Mol Engn, Beijing 100871, Peoples R China
2.Ruhr Univ Bochum, D-44780 Bochum, Germany
Recommended Citation
GB/T 7714
Lei, Rong,Stratmann, Lutz,Schaefer, Dominik,et al. Imaging Biocatalytic Activity of Enzyme-Polymer Spots by Means of Combined Scanning Electrochemical Microscopy/Electrogenerated Chemiluminescence[J]. ANALYTICAL CHEMISTRY,2009,81(12):5070-5074.
APA Lei, Rong.,Stratmann, Lutz.,Schaefer, Dominik.,Erichsen, Thomas.,Neugebauer, Sebastian.,...&Schuhmann, Wolfgang.(2009).Imaging Biocatalytic Activity of Enzyme-Polymer Spots by Means of Combined Scanning Electrochemical Microscopy/Electrogenerated Chemiluminescence.ANALYTICAL CHEMISTRY,81(12),5070-5074.
MLA Lei, Rong,et al."Imaging Biocatalytic Activity of Enzyme-Polymer Spots by Means of Combined Scanning Electrochemical Microscopy/Electrogenerated Chemiluminescence".ANALYTICAL CHEMISTRY 81.12(2009):5070-5074.
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