ICCAS OpenIR
Effect of refluxed silver nanoparticles on inhibition and enhancement of enzymatic activity of glucose oxidase
Ma, Shiyu1,2; Mu, Jin1; Qu, Yi2; Jiang, Long3
2009-08-05
Source PublicationCOLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
ISSN0927-7757
Volume345Issue:1-3Pages:101-105
AbstractGlucose oxidase was immobilized on the surface of silver nanoparticles. The enzymatic activity of the glucose oxidase was determined by the refluxing time of silver nanoparticles. In a short refluxing time, the silver nanoparticles behaved to inhibit the enzymatic activity of the glucose oxidase. However, the inhibitory effect of silver nanoparticles reduced along with the refluxing time. And an enhancement effect appeared as the silver nanoparticles were refluxed in a certain period of time. When the refluxing time of silver nanoparticles was 3.0 h, the enzymatic activity of the immobilized glucose oxidase reached the maximum value, which was almost 4-fold as compared with that of the native glucose oxidase. Fluorescence and circular dichroism spectra indicated that the glucose oxidase on the surface of silver nanoparticles changed its conformation, and the conformation change depended on the refluxing time of silver nanoparticles, which was probably due to different interactions between glucose oxidase and silver nanoparticles. (C) 2009 Elsevier B.V. All rights reserved.
KeywordGlucose Oxidase Inhibitory Effect Enhancement Effect Silver Nanoparticles Reflux Interaction
DOI10.1016/j.colsurfa.2009.04.038
Indexed BySCI
Language英语
WOS IDWOS:000268059800015
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/68488
Collection中国科学院化学研究所
Corresponding AuthorMu, Jin
Affiliation1.E China Univ Sci & Technol, Dept Chem, Key Lab Adv Mat, Shanghai 200237, Peoples R China
2.E China Normal Univ, Dept Chem, Shanghai 200062, Peoples R China
3.Chinese Acad Sci, Inst Chem, Key Lab Colloid & Interface Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Ma, Shiyu,Mu, Jin,Qu, Yi,et al. Effect of refluxed silver nanoparticles on inhibition and enhancement of enzymatic activity of glucose oxidase[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2009,345(1-3):101-105.
APA Ma, Shiyu,Mu, Jin,Qu, Yi,&Jiang, Long.(2009).Effect of refluxed silver nanoparticles on inhibition and enhancement of enzymatic activity of glucose oxidase.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,345(1-3),101-105.
MLA Ma, Shiyu,et al."Effect of refluxed silver nanoparticles on inhibition and enhancement of enzymatic activity of glucose oxidase".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 345.1-3(2009):101-105.
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