ICCAS OpenIR
[Ru(bpy)(2)dppz](2+) Electrochemiluminescence Switch and Its Applications for DNA Interaction Study and Label-free ATP Aptasensor
Hu, Lianzhe1,2; Bian, Zheng3; Li, Haijuan1,2; Han, Shuang1,2; Yuan, Yali1,2; Gao, Lianxun3; Xu, Guobao1
2009-12-01
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume81Issue:23Pages:9807-9811
Abstract[Ru(bpy)(2)dppz](2+) electrochemilumineseence (ECL) was studied, and it was used to investigate DNA interaction and develop a label-free ATP aptasensor for the first time. ECL of [Ru(bpy)(2)dppz](2+) is negligible in aqueous solution, and increases similar to 1000 times when [Ru(bpy)(2)dppz](2+) intercalates into the nucleic acid structure. The ECL switch behavior of [Ru(bpy)(2)dppz](2+) is ascribed to the intercalation that shields the phenazine nitrogens from the solvent and results in a luminescent excited state. The ECL switch by DNA was applied to investigate the interaction of [Ru(bpy)(2)dppz](2+) with herring sperm DNA. The calculated equilibrium constant (K) is 1.35 x 10(6) M-1, and the calculated binding-site size (s) is 0.88 base pair, which is consistent with the reported values. Moreover, ATP can dramatically affect ECL of the [Ru(bpy)(2)dppz](2+)/ATP aptamer complex. As a result, a label-free, sensitive, and selective [Ru(bpy)(2)dppz](2+) ECL method for ATP detection was developed. The detection limit is 100 nM for ATP (with a signal-to-noise ratio, S/N, of 3) with a linear range of 0-1 mu M. ne result demonstrates that [Ru(bpy)(2)dppz](2+) ECL holds great promise in aptasensors.
DOI10.1021/ac901925x
Indexed BySCI
Language英语
WOS IDWOS:000272084700043
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:117[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/68456
Collection中国科学院化学研究所
Corresponding AuthorXu, Guobao
Affiliation1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100864, Peoples R China
3.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Jilin, Peoples R China
Recommended Citation
GB/T 7714
Hu, Lianzhe,Bian, Zheng,Li, Haijuan,et al. [Ru(bpy)(2)dppz](2+) Electrochemiluminescence Switch and Its Applications for DNA Interaction Study and Label-free ATP Aptasensor[J]. ANALYTICAL CHEMISTRY,2009,81(23):9807-9811.
APA Hu, Lianzhe.,Bian, Zheng.,Li, Haijuan.,Han, Shuang.,Yuan, Yali.,...&Xu, Guobao.(2009).[Ru(bpy)(2)dppz](2+) Electrochemiluminescence Switch and Its Applications for DNA Interaction Study and Label-free ATP Aptasensor.ANALYTICAL CHEMISTRY,81(23),9807-9811.
MLA Hu, Lianzhe,et al."[Ru(bpy)(2)dppz](2+) Electrochemiluminescence Switch and Its Applications for DNA Interaction Study and Label-free ATP Aptasensor".ANALYTICAL CHEMISTRY 81.23(2009):9807-9811.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Hu, Lianzhe]'s Articles
[Bian, Zheng]'s Articles
[Li, Haijuan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Hu, Lianzhe]'s Articles
[Bian, Zheng]'s Articles
[Li, Haijuan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Hu, Lianzhe]'s Articles
[Bian, Zheng]'s Articles
[Li, Haijuan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.