ICCAS OpenIR
Analysis of local structure of Arg10 domain in apo-alpha-lactalbumin with a polarity-sensitive arginine-specific fluorescent probe
Wang ShuJuan; Chen SuMing; Ma HuiMin
2009-06-01
Source PublicationSCIENCE IN CHINA SERIES B-CHEMISTRY
ISSN1006-9291
Volume52Issue:6Pages:809-814
AbstractThe polarity-sensitive fluorescent probe, 3-(4-chloro-6-p-glyoxalphenoxy-1,3,5-triazinylamino)-7-(dimethylamino)-2-methylphenazine, was used to analyze the local structure of apo-alpha-lactalbumin by detecting the polarity and conformational changes of the arginine residue (Arg10) domain. The polarity of the Arg10 domain in both native and heat-denatured apo-alpha-lactalbumin was determined, which corresponds to a dielectric constant of 16, and the hydrophobic core near the Arg10 was found to be conservative for heating. Meanwhile, the effect of Ca(2+) binding on the conformational changes of the Arg10 domain was studied, revealing that the hydrophobic core near the Arg10 is insensitive to the binding of Ca(2+).
KeywordArginine-specific Labeling Fluorescent Probes Local Polarity Detection Conformational Changes Ca(2+) Binding
DOI10.1007/s11426-009-0027-x
Indexed BySCI
Language英语
WOS IDWOS:000266168000017
PublisherSCIENCE PRESS
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/68232
Collection中国科学院化学研究所
Corresponding AuthorMa HuiMin
AffiliationChinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Wang ShuJuan,Chen SuMing,Ma HuiMin. Analysis of local structure of Arg10 domain in apo-alpha-lactalbumin with a polarity-sensitive arginine-specific fluorescent probe[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2009,52(6):809-814.
APA Wang ShuJuan,Chen SuMing,&Ma HuiMin.(2009).Analysis of local structure of Arg10 domain in apo-alpha-lactalbumin with a polarity-sensitive arginine-specific fluorescent probe.SCIENCE IN CHINA SERIES B-CHEMISTRY,52(6),809-814.
MLA Wang ShuJuan,et al."Analysis of local structure of Arg10 domain in apo-alpha-lactalbumin with a polarity-sensitive arginine-specific fluorescent probe".SCIENCE IN CHINA SERIES B-CHEMISTRY 52.6(2009):809-814.
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