ICCAS OpenIR
Water-Soluble Conjugated Polyelectrolyte-Based Fluorescence Enzyme Coupling Protocol for Continuous and Sensitive beta-Galactosidase Detection
Feng, Fude; Liu, Libing; Wang, Shu
2009-08-06
Source PublicationMACROMOLECULAR CHEMISTRY AND PHYSICS
ISSN1022-1352
Volume210Issue:15Pages:1188-1193
AbstractA simple, rapid, continuous and homogeneous fluorescent assay for P-galactosidase was developed, combining an enzyme-coupled reaction and signal amplification property of conjugated polyelectrolytes (CPs). The procedure is based on a sequence of two coupled biocatalytic steps in which the beta-galactosidase hydrolyzes its substrate to a phenol derivative followed by conversion to quinone (secondary product) with fluorescence quenching ability by the tyrosinase. The fluorescence of PFP-SO(3)(-) is efficiently quenched by the quinone via an electron transfer process. The limit of detection (LOD) of this assay is less than 0.0005 U.mL(-1), which is better than that of electrochemical method and is comparable to that of most sensitive chemilumine scent techniques. In principle, this sensor mechanism will extend the application window of Us for wide-spectrum enzyme detections. This "mix-and-detect" approach could be expanded to a high-throughput manner.
KeywordEnzymes Fluorescence Polyelectrolytes Sensors Synthesis
DOI10.1002/macp.200900264
Indexed BySCI ; IC
Language英语
WOS IDWOS:000269175100001
PublisherWILEY-V C H VERLAG GMBH
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/67964
Collection中国科学院化学研究所
Corresponding AuthorLiu, Libing
AffiliationChinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Feng, Fude,Liu, Libing,Wang, Shu. Water-Soluble Conjugated Polyelectrolyte-Based Fluorescence Enzyme Coupling Protocol for Continuous and Sensitive beta-Galactosidase Detection[J]. MACROMOLECULAR CHEMISTRY AND PHYSICS,2009,210(15):1188-1193.
APA Feng, Fude,Liu, Libing,&Wang, Shu.(2009).Water-Soluble Conjugated Polyelectrolyte-Based Fluorescence Enzyme Coupling Protocol for Continuous and Sensitive beta-Galactosidase Detection.MACROMOLECULAR CHEMISTRY AND PHYSICS,210(15),1188-1193.
MLA Feng, Fude,et al."Water-Soluble Conjugated Polyelectrolyte-Based Fluorescence Enzyme Coupling Protocol for Continuous and Sensitive beta-Galactosidase Detection".MACROMOLECULAR CHEMISTRY AND PHYSICS 210.15(2009):1188-1193.
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