ICCAS OpenIR
Recognition of subtype non-small cell lung cancer by DNA aptamers selected from living cells
Zhao, Zilong1; Xu, Li1; Shi, Xiaoli1; Tan, Weihong2; Fang, Xiaohong1; Shangguan, Dihua1
2009
Source PublicationANALYST
ISSN0003-2654
Volume134Issue:9Pages:1808-1814
AbstractIn this work, we have developed new aptamer probes for non-small cell lung cancer (NSCLC) by directing the aptamer selection process against the living cells of adenocarcinoma, the most common subtype of NSCLC. A panel of single-stranded DNA (ssDNA) aptamers were generated and evaluated for adenocarcinoma cell recognition. The aptamers bound to the adenocarcinoma cells with dissociation constants in the nanomolar range and the binding of the selected aptamers to the adenocarcinoma cells were significantly stronger than the other cancerous lung cells as well as other types of cancer cells. Moreover, the application of the aptamers to the clinical tissue section samples showed the differentiation of adenocarcinoma from normal lung tissue and other subtypes of lung cancer. The aptamers are expected to be new molecular probes for the investigation of the molecular bases of different NSCLC subtypes and their biological heterogeneity, which is valuable for advancing NSCLC diagnosis and treatment.
DOI10.1039/b904476k
Indexed BySCI
Language英语
WOS IDWOS:000269063100010
PublisherROYAL SOC CHEMISTRY
Citation statistics
Cited Times:105[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/67828
Collection中国科学院化学研究所
Corresponding AuthorFang, Xiaohong
Affiliation1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
2.Univ Florida, Dept Chem, Shands Canc Ctr, Gainesville, FL 32601 USA
Recommended Citation
GB/T 7714
Zhao, Zilong,Xu, Li,Shi, Xiaoli,et al. Recognition of subtype non-small cell lung cancer by DNA aptamers selected from living cells[J]. ANALYST,2009,134(9):1808-1814.
APA Zhao, Zilong,Xu, Li,Shi, Xiaoli,Tan, Weihong,Fang, Xiaohong,&Shangguan, Dihua.(2009).Recognition of subtype non-small cell lung cancer by DNA aptamers selected from living cells.ANALYST,134(9),1808-1814.
MLA Zhao, Zilong,et al."Recognition of subtype non-small cell lung cancer by DNA aptamers selected from living cells".ANALYST 134.9(2009):1808-1814.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhao, Zilong]'s Articles
[Xu, Li]'s Articles
[Shi, Xiaoli]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhao, Zilong]'s Articles
[Xu, Li]'s Articles
[Shi, Xiaoli]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhao, Zilong]'s Articles
[Xu, Li]'s Articles
[Shi, Xiaoli]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.