Effect of functionalized micropatterned PLGA on guided neurite growth
Yao, Li1; Wang, Shenguo4; Cui, Wenjin4; Sherlock, Richard2; O'Connell, Claire2; Damodaran, Gopinath1; Gorman, Adrienne3; Windebank, Anthony5; Pandit, Abhay1
AbstractWhen coaptation is not possible in the repair of nerve injuries, a bridge of biomaterial scaffold provides a structural support for neuronal cell growth and guides nerve regeneration. Poly(lactide-co-glycolide) (PLGA) scaffolds have been widely investigated for neural tissue engineering applications. In order to investigate guided neurite growth, we have fabricated micropatterns on PLGA films using laser ablation methods. The micropatterned PLGA films were coated with collagen type I or laminin peptide (PPFLMLLKGSTR) to promote axon growth. Micropatterned PLGA films provide a guidance effect on both early stage neurite outgrowth and elongation. Small (5 mu m) grooves showed more statistically significant parallel neurite growth compared with larger size grooves (10 mu m). Micropatterned PLGA films coated with laminin peptide showed more parallel neurite growth compared with those coated with collagen type I. Primary neurite number and total neurite length per cell decreased oil micropatterned PLGA films compared with the controls. Neurites showed a preference for growth in the microgrooves rather than on the spaces. This study indicates that surface micropatterned structures with conjugated functional molecules can be used to guide neurite growth. (c) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
KeywordGuided Regeneration Micropatterning Laminin Peptide Plga
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WOS IDWOS:000262684600006
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Document Type期刊论文
Corresponding AuthorPandit, Abhay
Affiliation1.Natl Univ Ireland, Natl Ctr Biomed Engn Sci, Galway, Ireland
2.Natl Univ Ireland, Natl Ctr Laser Applicat, Galway, Ireland
3.Natl Univ Ireland, Dept Biochem, Galway, Ireland
4.Chinese Acad Sci, Inst Chem, State Key Lab Polymer Phys & Chem, BNLMS, Beijing 100080, Peoples R China
5.Mayo Clin, Dept Neurol, Rochester, MN USA
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GB/T 7714
Yao, Li,Wang, Shenguo,Cui, Wenjin,et al. Effect of functionalized micropatterned PLGA on guided neurite growth[J]. ACTA BIOMATERIALIA,2009,5(2):580-588.
APA Yao, Li.,Wang, Shenguo.,Cui, Wenjin.,Sherlock, Richard.,O'Connell, Claire.,...&Pandit, Abhay.(2009).Effect of functionalized micropatterned PLGA on guided neurite growth.ACTA BIOMATERIALIA,5(2),580-588.
MLA Yao, Li,et al."Effect of functionalized micropatterned PLGA on guided neurite growth".ACTA BIOMATERIALIA 5.2(2009):580-588.
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