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Formation of an Intramolecular G-Quadruplex of Human Telomere Induced by Poly(L-lysine) under Salt-Deficient Conditions
Zhang, Xiu-feng1; Xiang, Jun-feng1; Tian, Ming-yue1,2; Yang, Qian-fan1; Sun, Hong-xia1; Yang, Shu1; Tang, Ya-lin1
2009-05-28
Source PublicationJOURNAL OF PHYSICAL CHEMISTRY B
ISSN1520-6106
Volume113Issue:21Pages:7662-7667
AbstractA single-stranded G-tract human telomere DNA sequence is able to fold into intramolecular G-quadruplex structures which may be important for a number of biological processes and disease-related mechanisms. Poly(L-lysine) (PLL) polymer is linear polypeptides with lysine as the repeat unit and has been employed as a gene carrier in achieving targeted delivery of DNA to cancer cells. To explore the influence of PLL on the conformation of Hum24 DNA, we have investigated the interaction of PLL with Hum24 by biophysical methods, mainly CD, ESI-MS, and polyacrylamide gel electrophoresis for the first time. The CD data have shown that PLL can induce single-stranded Hum24 to form an intramolecular parallel G-quadruplex structure, further confirmed by ESI-MS analysis and gel electrophoresis results. The formation of an intramolecular G-quadruplex is strongly dependent on the Hum24/PLL molar ratios and the length of both the polypeptides and oligonucleotide. Such phenomena may be interpreted by electrostatically attracting negative-charged Hum24 by positive-charged PLL which facilitates the close contact between the guanines and formation of hydrogen bonding, thus leading the final shape of a G-quadruplex structure.
DOI10.1021/jp811183a
Indexed BySCI
Language英语
WOS IDWOS:000266296700034
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/67796
Collection中国科学院化学研究所
Corresponding AuthorTang, Ya-lin
Affiliation1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable and Stable Spe, Beijing 100190, Peoples R China
2.Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Xiu-feng,Xiang, Jun-feng,Tian, Ming-yue,et al. Formation of an Intramolecular G-Quadruplex of Human Telomere Induced by Poly(L-lysine) under Salt-Deficient Conditions[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2009,113(21):7662-7667.
APA Zhang, Xiu-feng.,Xiang, Jun-feng.,Tian, Ming-yue.,Yang, Qian-fan.,Sun, Hong-xia.,...&Tang, Ya-lin.(2009).Formation of an Intramolecular G-Quadruplex of Human Telomere Induced by Poly(L-lysine) under Salt-Deficient Conditions.JOURNAL OF PHYSICAL CHEMISTRY B,113(21),7662-7667.
MLA Zhang, Xiu-feng,et al."Formation of an Intramolecular G-Quadruplex of Human Telomere Induced by Poly(L-lysine) under Salt-Deficient Conditions".JOURNAL OF PHYSICAL CHEMISTRY B 113.21(2009):7662-7667.
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