ICCAS OpenIR
A label-free fluorescence sensor for probing the interaction of oligonucleotides with target molecules
Cheng, Xiaohong1,2; Bing, Tao1,2; Liu, Xiangjun1; Shangguan, Dihua1
2009-02-02
Source PublicationANALYTICA CHIMICA ACTA
ISSN0003-2670
Volume633Issue:1Pages:97-102
AbstractThis paper describes a universal, label-free fluorescence sensor that gauges the interaction of oligonucleotides with their targets. The sensor is based on supramolecular assemblies formed by oligonucleotides (polyanions) and small molecule cation surfactant in aqueous solution. The environmentally dependent dye pyrene, encapsulated in the apolar interiors of the assemblies via hydrophobic interactions works as the fluorescence probe. Target binding causes the conformational change of the oligonucleotides, which results in disorganization of supramolecular assemblies, release of pyrene into the aqueous solution and subsequent quenching of its fluorescence. The kinetic processes (including the formation of supramolecular assemblies and the release of pyrenes after adding the targets) were investigated. The fluorescence decreases of pyrenes are proportional to the concentrations of targets within the linear ranges. This label-free fluorescence system is simple,convenient, low cost, and can serve as an alternative tool for interaction studies of oligonucleotides with their targets, especially with small molecular targets. (C) 2008 Elsevier B.V. All rights reserved.
KeywordSupramolecular Assembly Fluorescence Sensor Oligonucleotide Ligand Molecular Interaction
DOI10.1016/j.aca.2008.11.031
Indexed BySCI
Language英语
WOS IDWOS:000262734700013
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/67776
Collection中国科学院化学研究所
Corresponding AuthorShangguan, Dihua
Affiliation1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Cheng, Xiaohong,Bing, Tao,Liu, Xiangjun,et al. A label-free fluorescence sensor for probing the interaction of oligonucleotides with target molecules[J]. ANALYTICA CHIMICA ACTA,2009,633(1):97-102.
APA Cheng, Xiaohong,Bing, Tao,Liu, Xiangjun,&Shangguan, Dihua.(2009).A label-free fluorescence sensor for probing the interaction of oligonucleotides with target molecules.ANALYTICA CHIMICA ACTA,633(1),97-102.
MLA Cheng, Xiaohong,et al."A label-free fluorescence sensor for probing the interaction of oligonucleotides with target molecules".ANALYTICA CHIMICA ACTA 633.1(2009):97-102.
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