ICCAS OpenIR
Analysis of local polarity change around Cys34 in bovine serum albumin during N -> B transition by a polarity-sensitive fluorescence probe
Wang, Xiaochun1,2; Guo, Lihong3; Ma, Huimin1
2009-09-01
Source PublicationSPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
ISSN1386-1425
Volume73Issue:5Pages:875-878
AbstractThe change trend of the local environment of Cys34 domain in bovine serum albumin has been studied as a function of pH value by using thiol-specific and polarity-sensitive fluorescent probe 3-(4-chloro-6-p-maleimidylphenoxyl-1,3,5-triazinylamino)-7-dimethylamino-2-methyl-phenazine. The local polarity of the Cys34 domain is found to rise with the increase of pH values, and the corresponding dielectric constant is raised from 12.8 at pH 6.0 to 23.3 at pH 9.1. The result shows that the environment of the Cys34 domain is rather hydrophobic in normal state at pH 6.0 and becomes a little hydrophilic in the course of N -> B transition, which may be attributed to the slight unfolding of the protein and thus the increasing of exposure of the previously relatively buried Cys34. In addition, the increased dielectric constant (23.3) is much lower than that (80.1) of water, suggesting that the unfolding of bovine serum albumin does not cause the full exposure of the Cys34 to the aqueous media during the transition. (C) 2009 Elsevier B.V. All rights reserved.
KeywordBovine Serum Albumin n -> b Transition Cys34 Domain Local Polarity Fluorescence Probe
DOI10.1016/j.saa.2009.04.008
Indexed BySCI
Language英语
WOS IDWOS:000268009400015
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/67706
Collection中国科学院化学研究所
Corresponding AuthorMa, Huimin
Affiliation1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
2.Natl Res Ctr Geoanal, Beijing 100037, Peoples R China
3.Beijing Mil Area Command, Joint Logitist Dept, Inst Drug & Instrument Control, Beijing 100071, Peoples R China
Recommended Citation
GB/T 7714
Wang, Xiaochun,Guo, Lihong,Ma, Huimin. Analysis of local polarity change around Cys34 in bovine serum albumin during N -> B transition by a polarity-sensitive fluorescence probe[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2009,73(5):875-878.
APA Wang, Xiaochun,Guo, Lihong,&Ma, Huimin.(2009).Analysis of local polarity change around Cys34 in bovine serum albumin during N -> B transition by a polarity-sensitive fluorescence probe.SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,73(5),875-878.
MLA Wang, Xiaochun,et al."Analysis of local polarity change around Cys34 in bovine serum albumin during N -> B transition by a polarity-sensitive fluorescence probe".SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 73.5(2009):875-878.
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