ICCAS OpenIR
A Biomimetic Potassium Responsive Nanochannel: G-Quadruplex DNA Conformational Switching in a Synthetic Nanopore
Hou, Xu3; Guo, Wei1; Xia, Fan2; Nie, Fu-Qiang2; Dong, Hua2; Tian, Ye2; Wen, Liping2; Wang, Lin1; Cao, Liuxuan1; Yang, Yang3; Xue, Jianming1; Song, Yanlin2; Wang, Yugang1; Liu, Dongsheng3; Jiang, Lei2
2009-06-10
Source PublicationJOURNAL OF THE AMERICAN CHEMICAL SOCIETY
ISSN0002-7863
Volume131Issue:22Pages:7800-7805
AbstractPotassium is especially crucial in modulating the activity of muscles and nerves whose cells have specialized ion channels for transporting potassium. Normal body function extremely depends on the regulation of potassium concentrations inside the ion channels within a certain range. For life science, undoubtedly, it is significant and challenging to study and imitate these processes happening in living organisms with a convenient artificial system. Here we report a novel biomimetic nanochannel system which. has an ion concentration effect that provides a nonlinear response to potassium ion at the concentration ranging from 0 to 1500 mu M. This new phenomenon is caused by the G-quadruplex DNA conformational change with a positive correlation with ion concentration. In this work, G-quadruplex DNA was immobilized onto a synthetic nanopore, which undergoes a potassium-responsive conformational change and then induces the change in the effective pore size. The responsive ability of this system can be regulated by the stability of G-quadruplex structure through adjusting potassium concentration. The situation of the grafting G-quadruplex DNA on a single nanopore can closely imitate the in vivo condition because the G-rich telomere overhang is attached to the chromosome. Therefore, this artificial system could promote a potential to conveniently study biomolecule conformational change in confined space by the current measurement, which is significantly different from the nanopore sequencing. Moreover, such a system may also potentially spark further experimental and theoretical efforts to simulate the process of ion transport in living organisms and can be further generalized to other more complicated functional molecules for the exploitation of novel bioinspired intelligent nanopore machines.
DOI10.1021/ja901574c
Indexed BySCI
Language英语
WOS IDWOS:000267177900070
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:237[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/67696
Collection中国科学院化学研究所
Corresponding AuthorWang, Yugang
Affiliation1.Peking Univ, State Key Lab Nucl Phys & Technol, Beijing 100871, Peoples R China
2.Chinese Acad Sci, Ctr Mol Sci, Inst Chem, Beijing 100190, Peoples R China
3.Natl Ctr Nanosci & Nanotechnol, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Hou, Xu,Guo, Wei,Xia, Fan,et al. A Biomimetic Potassium Responsive Nanochannel: G-Quadruplex DNA Conformational Switching in a Synthetic Nanopore[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2009,131(22):7800-7805.
APA Hou, Xu.,Guo, Wei.,Xia, Fan.,Nie, Fu-Qiang.,Dong, Hua.,...&Jiang, Lei.(2009).A Biomimetic Potassium Responsive Nanochannel: G-Quadruplex DNA Conformational Switching in a Synthetic Nanopore.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,131(22),7800-7805.
MLA Hou, Xu,et al."A Biomimetic Potassium Responsive Nanochannel: G-Quadruplex DNA Conformational Switching in a Synthetic Nanopore".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 131.22(2009):7800-7805.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Hou, Xu]'s Articles
[Guo, Wei]'s Articles
[Xia, Fan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Hou, Xu]'s Articles
[Guo, Wei]'s Articles
[Xia, Fan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Hou, Xu]'s Articles
[Guo, Wei]'s Articles
[Xia, Fan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.