ICCAS OpenIR
Analysis of aristolochic acids by CE-MS with carboxymethyl chitosan-coated capillary
Fu, Xiaofang1; Liu, Yi1; Li, Wei1; Pang, Nannan1; Nie, Honggang1; Liu, Huwei1; Cai, Zongwei2
2009-05-01
Source PublicationELECTROPHORESIS
ISSN0173-0835
Volume30Issue:10Pages:1783-1789
AbstractA CE-MS method for rapid determination of aristolochic acid-I and aristolochic acid-II (AA-II) in traditional Chinese medicines and biological samples was described in the present paper. AA-I and AA-II can be baseline separated within 6 min by CE-MS with carboxymethyl-chitosan-coated capillary. CZE conditions including pH, concentration of buffer, applied voltage, and capillary temperature were systematically investigated, and the composition and flow rate of sheath liquid were also optimized for CE-MS. Furthermore, the CE-UV method without any additives in BGE solution was established and compared with the CE-MS method. The results showed that the two methods could achieve satisfactory separation efficiency, repeatability, and linearity, while the LOD was 0.6 mu g/mL for CE-UV and 0.05 mu g/mL for CE-MS. Compared with the CE-UV method, the sensitivity of CE-MS was significantly improved, in addition to the structure information provided by MS detection at the same time. As an application example, a spiked sample in human serum was analyzed by the CE-MS method, indicating that the new CE-MS method can be applied to analyze AAs in biological samples.
KeywordAristolochic Acid Capillary Coating Carboxymethyl Chitosan Ce Ms
DOI10.1002/elps.200800487
Indexed BySCI
Language英语
WOS IDWOS:000266524500020
PublisherWILEY-V C H VERLAG GMBH
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/67668
Collection中国科学院化学研究所
Corresponding AuthorLiu, Huwei
Affiliation1.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci,Inst Analyt Chem, Key Lab Bioorgan Chem & Mol Engn,Minist Educ, Beijing 100871, Peoples R China
2.Hong Kong Baptist Univ, Dept Chem, Hong Kong, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Fu, Xiaofang,Liu, Yi,Li, Wei,et al. Analysis of aristolochic acids by CE-MS with carboxymethyl chitosan-coated capillary[J]. ELECTROPHORESIS,2009,30(10):1783-1789.
APA Fu, Xiaofang.,Liu, Yi.,Li, Wei.,Pang, Nannan.,Nie, Honggang.,...&Cai, Zongwei.(2009).Analysis of aristolochic acids by CE-MS with carboxymethyl chitosan-coated capillary.ELECTROPHORESIS,30(10),1783-1789.
MLA Fu, Xiaofang,et al."Analysis of aristolochic acids by CE-MS with carboxymethyl chitosan-coated capillary".ELECTROPHORESIS 30.10(2009):1783-1789.
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