ICCAS OpenIR
Continuous Colorimetric Assay for Acetylcholinesterase and Inhibitor Screening with Gold Nanoparticles
Wang, Ming; Gu, Xinggui; Zhang, Guanxin; Zhang, Deqing; Zhu, Daoben
2009-02-17
Source PublicationLANGMUIR
ISSN0743-7463
Volume25Issue:4Pages:2504-2507
AbstractWe report herein a new colorimetric assay method for acetylcholinesterase (AChE) activity and its inhibitor screening by making use of the following facts: (1) the aggregation of gold nanoparticles (Au-NPs) results in the red-shift of the plasmon absorption due to interparticle plasmon interactions and (2) AChE can catalyze the hydrolysis of acetylthiocholine into thiocholine which can induce the aggregation of Au-NPs. With this convenient method, the activity of AChE with a concentration as low as 0.6 mU/mL can be assayed. Moreover, this assay method is also useful for screening inhibitors of AChE. Given its simplicity and easy-operation, this method may extend to high-throughput screening of AChE inhibitors and relevant drug discovery.
DOI10.1021/la803870v
Indexed BySCI
Language英语
WOS IDWOS:000263373600089
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:116[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/67654
Collection中国科学院化学研究所
Corresponding AuthorZhang, Deqing
AffiliationChinese Acad Sci, Inst Chem, Organ Solids Lab, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Wang, Ming,Gu, Xinggui,Zhang, Guanxin,et al. Continuous Colorimetric Assay for Acetylcholinesterase and Inhibitor Screening with Gold Nanoparticles[J]. LANGMUIR,2009,25(4):2504-2507.
APA Wang, Ming,Gu, Xinggui,Zhang, Guanxin,Zhang, Deqing,&Zhu, Daoben.(2009).Continuous Colorimetric Assay for Acetylcholinesterase and Inhibitor Screening with Gold Nanoparticles.LANGMUIR,25(4),2504-2507.
MLA Wang, Ming,et al."Continuous Colorimetric Assay for Acetylcholinesterase and Inhibitor Screening with Gold Nanoparticles".LANGMUIR 25.4(2009):2504-2507.
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