ICCAS OpenIR
Physiologically Relevant Online Electrochemical Method for Continuous and Simultaneous Monitoring of Striatum Glucose and Lactate Following Global Cerebral Ischemia/Reperfusion
Lin, Yuqing; Zhu, Ningning; Yu, Ping; Su, Lei; Mao, Lanqun
2009-03-15
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume81Issue:6Pages:2067-2074
AbstractThis study demonstrates a new electroanalytical method with a high physiological relevance for simultaneous online monitoring of glucose and lactate in the striatum of the rat brain following global cerebral ischemia/reperfusion. The online analytical method is based on the efficient integration of in vivo microdialysis sampling with an online selective electrochemical detection with the electrochemical biosensors with dehydrogenases, i.e., glucose and lactate dehydrogenases, as recognition elements. The dehydrogenase-based electrochemical biosensors are developed onto the dual split-disk plastic carbon film (SPCF) electrodes with methylene green (MG) adsorbed onto single-walled carbon nanotubes (SWNTs) as the electrocatalyst for the oxidation of dihydronicotiamide adenine dinucleotide (NADH) at a low potential of 0.0 V (vs Ag/AgCl). Artificial cerebrospinal fluid (aCSF) containing NAD(+) is externally perfused from a second pump and online mixed with the brain microdialysates to minimize the variation of pH that occurred following the cerebral ischemia/reperfusion and to supply NAD(+) cofactor and O(2) for the enzymatic reactions of dehydrogenases and ascorbate oxidase, respectively. As a result, the developed online electroanalytical method exhibits a high selectivity against the electrochemically active species endogenously existing in the cerebral systems and a high tolerance against the variation of pH and O(2) following cerebral ischemia/reperfusion. This property, along with the good linearity and a high stability toward glucose and lactate as well as little cross-talk between two biosensors, substantially makes this method possible for the continuous, simultaneous, and online monitoring of glucose and lactate in the rat brain following global cerebral ischemia/reperfusion. This study establishes a new and effective platform for the investigation of the energy metabolism in physiological and pathological processes.
DOI10.1021/ac801946s
Indexed BySCI
Language英语
WOS IDWOS:000264142000005
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:85[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/67650
Collection中国科学院化学研究所
Corresponding AuthorMao, Lanqun
AffiliationChinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Lin, Yuqing,Zhu, Ningning,Yu, Ping,et al. Physiologically Relevant Online Electrochemical Method for Continuous and Simultaneous Monitoring of Striatum Glucose and Lactate Following Global Cerebral Ischemia/Reperfusion[J]. ANALYTICAL CHEMISTRY,2009,81(6):2067-2074.
APA Lin, Yuqing,Zhu, Ningning,Yu, Ping,Su, Lei,&Mao, Lanqun.(2009).Physiologically Relevant Online Electrochemical Method for Continuous and Simultaneous Monitoring of Striatum Glucose and Lactate Following Global Cerebral Ischemia/Reperfusion.ANALYTICAL CHEMISTRY,81(6),2067-2074.
MLA Lin, Yuqing,et al."Physiologically Relevant Online Electrochemical Method for Continuous and Simultaneous Monitoring of Striatum Glucose and Lactate Following Global Cerebral Ischemia/Reperfusion".ANALYTICAL CHEMISTRY 81.6(2009):2067-2074.
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