ICCAS OpenIR
Preparation of poly(N-isopropylacrylamide)-grafted polymer monolith for hydrophobic interaction chromatography of proteins
Zhang, Rongyue1,2; Yang, Gengliang1,3; Xin, Peiyong1,2; Qi, Li1; Chen, Yi1
2009-03-20
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1216Issue:12Pages:2404-2411
AbstractPoly(N-isopropylacrylamide)-grafted polymer monolith has been achieved using a surface-initiated atom transfer radical polymerization grafting polymerization within the pores of poly(chloromethylstyrene-divinylbenzene) macroporous monolith contained in a 100 mm x 4.6 mm I.D. stainless steel column. The grafted-poly(N-isopropylacrylamide) on the surface of the grafted monolith that was used as chromatographic stationary phase showed a response to the variation of temperatures and/or salt concentrations. This study focus on its salt concentration responsive property and it has been revealed that the hydrophobicity of the grafted monolith can be adjusted by changing salt concentrations in the range of 0.05-2.0 rnol/L. A variety of salts including sodium sulfate, ammonium sulfate and sodium chloride exhibited different effects on the alteration of hydrophobicity of the grafted monolith, and the effect of the salts was in the order of sodium sulfate > ammonium sulfate > sodium chloride. Based on this response to salt concentrations, the grafted monolith was applied in hydrophobic interaction chromatography of proteins, and the base-line separation of a six proteins mixture consisting of cytochrome c, myoglobin, ribonuclease A, bovine serum albumin, ovalbumin and thyroglobulin bovine was achieved by a salt gradient elution. (c) 2009 Elsevier B.V. All rights reserved.
KeywordHydrophobic Interaction Chromatography Proteins Poly(N-isopropylacrylamide) Response To Salt Concentration Surface-initiated Atom Transfer Radical Polymerization Monolithic Column
DOI10.1016/j.chroma.2009.01.023
Indexed BySCI
Language英语
WOS IDWOS:000264217800025
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:41[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/67620
Collection中国科学院化学研究所
Corresponding AuthorYang, Gengliang
Affiliation1.Chinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Hebei Univ, Coll Pharm, Baoding 071002, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Rongyue,Yang, Gengliang,Xin, Peiyong,et al. Preparation of poly(N-isopropylacrylamide)-grafted polymer monolith for hydrophobic interaction chromatography of proteins[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(12):2404-2411.
APA Zhang, Rongyue,Yang, Gengliang,Xin, Peiyong,Qi, Li,&Chen, Yi.(2009).Preparation of poly(N-isopropylacrylamide)-grafted polymer monolith for hydrophobic interaction chromatography of proteins.JOURNAL OF CHROMATOGRAPHY A,1216(12),2404-2411.
MLA Zhang, Rongyue,et al."Preparation of poly(N-isopropylacrylamide)-grafted polymer monolith for hydrophobic interaction chromatography of proteins".JOURNAL OF CHROMATOGRAPHY A 1216.12(2009):2404-2411.
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