ICCAS OpenIR
Construction of Effective Receptor for Recognition of Avian Influenza H5N1 Protein HA1 by Assembly of Monohead Glycolipids on Polydiacetylene Vesicle Surface
Deng, Jieli1; Sheng, Zhonghan1; Zhou, Kai2; Duan, Mingxing2; Yu, Chu-yi1; Jiang, Long1
2009-03-01
Source PublicationBIOCONJUGATE CHEMISTRY
ISSN1043-1802
Volume20Issue:3Pages:533-537
AbstractFast and sensitive detection of epidemic virus is of the utmost importance for human being in nowadays. Various biosensors have been designed for this goal based on conjugation event between host cell glycolipids and invading virus. However, multihead glycolipids analogous to native receptors on cell surface are known to be very difficult to mimic because of the complexity of chemical synthesis. Here, we developed a new approach where two types of monohead glycolipids, active sialic acid-beta-glucoside (G1) and inactive lactose-beta-glucoside (G2), are embedded onto the surface of a polydiacetylene (PDA) vesicle to mimic native glycolipids on the cell surface. Vesicles prepared in this manner show good selectivity with a 10 ng/mL detection limit and 5 min response time to Hemagglutinin (HA1), which is more sensitive than any HA1 biosensors ever known. Moreover, in the formation of color-changeable vesicles, a very strong synergistic effect between G1 and G2 has been found, offering a novel strategy to construct effective biosensor receptors, as well as a new way to study the surface combination effect that is potentially important to the immunology study of epidemic disease.
DOI10.1021/bc800453u
Indexed BySCI
Language英语
WOS IDWOS:000264389800017
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:40[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/67524
Collection中国科学院化学研究所
Corresponding AuthorJiang, Long
Affiliation1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
2.Tsinghua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
Recommended Citation
GB/T 7714
Deng, Jieli,Sheng, Zhonghan,Zhou, Kai,et al. Construction of Effective Receptor for Recognition of Avian Influenza H5N1 Protein HA1 by Assembly of Monohead Glycolipids on Polydiacetylene Vesicle Surface[J]. BIOCONJUGATE CHEMISTRY,2009,20(3):533-537.
APA Deng, Jieli,Sheng, Zhonghan,Zhou, Kai,Duan, Mingxing,Yu, Chu-yi,&Jiang, Long.(2009).Construction of Effective Receptor for Recognition of Avian Influenza H5N1 Protein HA1 by Assembly of Monohead Glycolipids on Polydiacetylene Vesicle Surface.BIOCONJUGATE CHEMISTRY,20(3),533-537.
MLA Deng, Jieli,et al."Construction of Effective Receptor for Recognition of Avian Influenza H5N1 Protein HA1 by Assembly of Monohead Glycolipids on Polydiacetylene Vesicle Surface".BIOCONJUGATE CHEMISTRY 20.3(2009):533-537.
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