ICCAS OpenIR
Enantioseparation of dansyl amino acids by ligand-exchange capillary electrophoresis with zinc(II)-L-phenylalaninamide complex
Qi, Li1,2; Yang, Gengliang1
2009-09-01
Source PublicationJOURNAL OF SEPARATION SCIENCE
ISSN1615-9306
Volume32Issue:18Pages:3209-3214
AbstractA novel method of chiral ligand-exchange CE was developed with L-amino acylamides as a chiral ligand and zinc(II) as a central ion. It has been demonstrated that these chiral complexes, such as Zn(II)-L-alaninamide, Zn(II)-L-prolinamide, and Zn(II)-L-phenylalaninamide, are suitable for use as chiral selectors for the enantioseparation of either individual pair of or mixed dansyl amino acids. The optimal separation running buffer consisted of 5 mM ammonium acetate, 100 mM boric acid, 4 mM ZnSO(4) center dot 7H(2)O, and 8 mM L-amino acylamides at pH 8.2. The experiments showed that apart from the effect of the concentration of the complexes on the resolution and the migration time, the buffer pH also had a sharp influence on resolution. The employed chiral ligands exhibited different enantioselectivities and enantiomer migration orders. D-Amino acids migrate faster than L-amino acids when Zn(II)-L-alaninamide and Zn(II)-L-phenylalaninamide are used as chiral selectors, but it was observed that the migration order is reversed when Zn(II)-L-prolinamide is used as the chiral selector. Furthermore, the amount of dansylated amino acids is found to be highly dependent on the labeling temperature.
KeywordDansyl Amino Acid Enantioseparation Ligand-exchange Capillary Electrophoresis Zinc-l-phenylalaninamide Complex
DOI10.1002/jssc.200900328
Indexed BySCI
Language英语
WOS IDWOS:000270375300018
PublisherWILEY-V C H VERLAG GMBH
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/67076
Collection中国科学院化学研究所
Corresponding AuthorYang, Gengliang
Affiliation1.Hebei Univ, Coll Pharmaceut Sci, Baoding 071002, Peoples R China
2.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Lab Analyt Chem Life Sci, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Qi, Li,Yang, Gengliang. Enantioseparation of dansyl amino acids by ligand-exchange capillary electrophoresis with zinc(II)-L-phenylalaninamide complex[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(18):3209-3214.
APA Qi, Li,&Yang, Gengliang.(2009).Enantioseparation of dansyl amino acids by ligand-exchange capillary electrophoresis with zinc(II)-L-phenylalaninamide complex.JOURNAL OF SEPARATION SCIENCE,32(18),3209-3214.
MLA Qi, Li,et al."Enantioseparation of dansyl amino acids by ligand-exchange capillary electrophoresis with zinc(II)-L-phenylalaninamide complex".JOURNAL OF SEPARATION SCIENCE 32.18(2009):3209-3214.
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