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Probing tyrosine Z oxidation in photosystem II core complex isolated from spinach by EPR at liquid helium temperatures
Ren, Yanan1; Zhang, Chunxi1; Bao, Han1; Shen, Jianren2; Zhao, Jingquan1
2009-02-01
Source PublicationPHOTOSYNTHESIS RESEARCH
ISSN0166-8595
Volume99Issue:2Pages:127-138
AbstractTyrosine Z (Tyr(Z)) oxidation observed at liquid helium temperatures provides new insights into the structure and function of Tyr(Z) in active Photosystem II (PSII). However, it has not been reported in PSII core complex from higher plants. Here, we report Tyr(Z) oxidation in the S(1) and S(2) states in PSII core complex from spinach for the first time. Moreover, we identified a 500 G-wide symmetric EPR signal (peak position g = 2.18, trough position g = 1.85) together with the g = 2.03 signal induced by visible light at 10 K in the S(1) state in the PSII core complex. These two signals decay with a similar rate in the dark and both disappear in the presence of 6% methanol. We tentatively assign this new feature to the hyperfine structure of the S(1)Tyr(Z) (aEuro cent) EPR signal. Furthermore, EPR signals of the S(2) state of the Mn-cluster, the oxidation of the non-heme iron, and the S(1)Tyr(Z) (aEuro cent) in PSII core complexes and PSII-enriched membranes from spinach are compared, which clearly indicate that both the donor and acceptor sides of the reaction center are undisturbed after the removal of LHCII. These results suggest that the new spinach PSII core complex is suitable for the electron transfer study of PSII at cryogenic temperatures.
KeywordPhotosystem Ii Core Complex Tyrosine z Spinach Epr
DOI10.1007/s11120-009-9410-x
Indexed BySCI
Language英语
WOS IDWOS:000263544900006
PublisherSPRINGER
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/66906
Collection中国科学院化学研究所
Corresponding AuthorZhang, Chunxi
Affiliation1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Lab Photochem, Beijing 100190, Peoples R China
2.Okayama Univ, Grad Sch Nat Sci & Technol, Fac Sci, Dept Biol, Okayama 7008530, Japan
Recommended Citation
GB/T 7714
Ren, Yanan,Zhang, Chunxi,Bao, Han,et al. Probing tyrosine Z oxidation in photosystem II core complex isolated from spinach by EPR at liquid helium temperatures[J]. PHOTOSYNTHESIS RESEARCH,2009,99(2):127-138.
APA Ren, Yanan,Zhang, Chunxi,Bao, Han,Shen, Jianren,&Zhao, Jingquan.(2009).Probing tyrosine Z oxidation in photosystem II core complex isolated from spinach by EPR at liquid helium temperatures.PHOTOSYNTHESIS RESEARCH,99(2),127-138.
MLA Ren, Yanan,et al."Probing tyrosine Z oxidation in photosystem II core complex isolated from spinach by EPR at liquid helium temperatures".PHOTOSYNTHESIS RESEARCH 99.2(2009):127-138.
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