ICCAS OpenIR
Ultrasensitive immunoassay of 7-aminoclonazepam in human urine based on CdTe nanoparticle bioconjugations by fabricated microfluidic chip
Chen, Wei2,3; Peng, Chifang2,3; Jin, Zhengyue2,3; Qiao, Ruirui4; Wang, Wuyang5; Zhu, Shuifang6; Wang, Libing7; Jing, Qinhui1; Xu, Chuanlai2,3
2009-03-15
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
Volume24Issue:7Pages:2051-2056
AbstractThe present paper described a rapid and ultrasensitive detection method using a microfluidic chip for analyzing 7-aminoclonazepam (7-ACZP) residues in human urine. A microfluidic chip-based immunoassay with laser-induced fluorescence (LIF) detection based on the water-soluble denatured bovine serum albumin (dBSA)-coated CdTe quantum dots (QDs) was prepared for the ultrasensitive detection of 7-ACZP. The whole procedure including the chip and the control software was designed and constructed in our own laboratory. The detection of 7-ACZP could be completed within 5 min. The results demonstrated that under the optima conditions, 7-ACZP residues could be detected with a precision of 5% relative standard deviation (RSD), and the linear range and the limit of detection (LOD) for 7-ACZP were 1.1-60.1 and 0.021 ng mL(-1), respectively. This method was compared with ELISA and showed a good correlation. This microfluidic chip with LIF detection was applied to the determination of 7-ACZP residues in positive human urine samples, and the results were confirmed by high-performance liquid chromatography and tandem mass spectrometry (LC/MS/MS). This ultrasensitive detection technique was proved to be practical for clinical use. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.
KeywordUltrasensitive Immunoassay 7-aminoclonazepam Cdte Nanoparticle Microfluidic Chip Lc/ms/ms
DOI10.1016/j.bios.2008.10.015
Indexed BySCI
Language英语
WOS IDWOS:000264791000039
PublisherELSEVIER ADVANCED TECHNOLOGY
Citation statistics
Cited Times:37[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/66426
Collection中国科学院化学研究所
Corresponding AuthorJing, Qinhui
Affiliation1.Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, Shanghai 200050, Peoples R China
2.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China
3.Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China
4.CAS, Inst Chem, Beijing 10008, Peoples R China
5.Fourth Hosp Wuxi, Wuxi 214036, Peoples R China
6.Chinese Acad Inspect & Quarantine, Beijing 10008, Peoples R China
7.Tianjin Acad Inspect & Quarantine, Tianjin 300457, Peoples R China
Recommended Citation
GB/T 7714
Chen, Wei,Peng, Chifang,Jin, Zhengyue,et al. Ultrasensitive immunoassay of 7-aminoclonazepam in human urine based on CdTe nanoparticle bioconjugations by fabricated microfluidic chip[J]. BIOSENSORS & BIOELECTRONICS,2009,24(7):2051-2056.
APA Chen, Wei.,Peng, Chifang.,Jin, Zhengyue.,Qiao, Ruirui.,Wang, Wuyang.,...&Xu, Chuanlai.(2009).Ultrasensitive immunoassay of 7-aminoclonazepam in human urine based on CdTe nanoparticle bioconjugations by fabricated microfluidic chip.BIOSENSORS & BIOELECTRONICS,24(7),2051-2056.
MLA Chen, Wei,et al."Ultrasensitive immunoassay of 7-aminoclonazepam in human urine based on CdTe nanoparticle bioconjugations by fabricated microfluidic chip".BIOSENSORS & BIOELECTRONICS 24.7(2009):2051-2056.
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