ICCAS OpenIR
Gemini Surfactant-Induced DNA Condensation into a Beadlike Structure
Wang, Xiao-Ling1; Zhang, Xing-Hua1; Cao, Meiwen2; Zheng, Hai-Zi1; Xiao, Bin1; Wang, Yilin2; Li, Ming1
2009-02-26
Source PublicationJOURNAL OF PHYSICAL CHEMISTRY B
ISSN1520-6106
Volume113Issue:8Pages:2328-2332
AbstractUsing a magnetic tweezers (MT) apparatus and an atomic force microscope (AFM), we studied the condensation of DNA induced by the cationic gemini surfactant hexyl-alpha,omega-bisdodecyidimethylammonium bromide (C(12)C(6)C(12)Br(2)). Stepwise condensation events were found for forces from 0.5 to 4 pN, with a decrease in DNA extension by similar to 100 nm in each condensation event. Applied stretching forces larger than 6 pN were found to be able to decondense the condensates in a similar intermittent stepwise manner, but with similar to 60 nm of DNA released in each decondensation event. These observations are consistent with AFM images that show beadlike structures on DNA. The results lead to a model in which, during condensation, independent beadlike structures are initially formed on DNA, and as the local density of the beads increases, they compact into higher-order structures while maintaining their independence. The condensation process is different from any previously reported condensation process, but it is somewhat similar to that of chromatin assembly.
DOI10.1021/jp8078887
Indexed BySCI
Language英语
WOS IDWOS:000263529500014
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:30[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iccas.ac.cn/handle/121111/66414
Collection中国科学院化学研究所
Corresponding AuthorLi, Ming
Affiliation1.Chinese Acad Sci, Beijing Natl Lab Condensed Matter Phys, Inst Phys, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Key Lab Colloid & Interface Sci, Inst Chem, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Wang, Xiao-Ling,Zhang, Xing-Hua,Cao, Meiwen,et al. Gemini Surfactant-Induced DNA Condensation into a Beadlike Structure[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2009,113(8):2328-2332.
APA Wang, Xiao-Ling.,Zhang, Xing-Hua.,Cao, Meiwen.,Zheng, Hai-Zi.,Xiao, Bin.,...&Li, Ming.(2009).Gemini Surfactant-Induced DNA Condensation into a Beadlike Structure.JOURNAL OF PHYSICAL CHEMISTRY B,113(8),2328-2332.
MLA Wang, Xiao-Ling,et al."Gemini Surfactant-Induced DNA Condensation into a Beadlike Structure".JOURNAL OF PHYSICAL CHEMISTRY B 113.8(2009):2328-2332.
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